The Microbiota Assays developed by AnyGenes® have been optimised with it range of Perfect Master Mix SYBR® Green to offer you a robust real-time qPCR system providing highly specific, reliable and reproducible results, even with small amounts of biological material, whatever the source of your samples.
All our microbiota assays are validated at the experimental level on a large collection of microbial DNA on our high-throughput molecular platform, according to stringent and strong quality control criteria. After validation in qPCR, each PCR amplicon is sequenced to ensure of the strict specificity of our microbiota primer sets to a unique microbial strain, contrary to 16S qPCR strategy that may induce non-specific amplification, due to highly conserved regions.
Our assays contains several internal controls to assess the high performance of the Microbiota Assays or SignArrays®, the quality of your samples, and the absence of DNA contamination.
These ready-to-use microbiota reagents are compatible with:
- all sample types: gut, cutaneous, vaginal flora...
- all host species: Homo sapiens, laboratory models like Mus musculus or Rattus norvegicus...
- all qPCR instruments
- all microbiota application fields: involvement in cancer, immune system regulation, inflammation, homeostasis and antibiotic resistance.
Several formats are proposed (microbiota assays, Personalized Microbiota SignArrays® 96 or 384 plates), to better answer to each of your project issues.
Microbiota individual assays prices
Reference type : MB-XXXX-100 | |
Microbiota individual assays | |
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*XXXX = microbial species name (according to the NCBI Taxonomy Browser approved nomenclature)
(see Compatibility file)* required number of reactions
* MB for Microbiota
Example: ME-B.vulgatus.8482-100 for the Bacteroides vulgatus ATCC 8482 species for 100 reactions
Microbiota SignArrays® 96 system prices
Reference type : PZxHMB1-Y2S | |
Customizable | |
Number of SignArrays® 96 |
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* Y: R, A, B or F plate type, according to the qPCR instrument (cf Compatibility file)
(see Compatibility file)*2: according to the number of ordered Microbiota SignArrays®
example: PZxHMB1-R12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument
NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.
Microbiota SignArrays® 384 system prices
Reference type : PZxHMB2-Y2S | |
Customizable | |
Number of SignArrays® 384 |
Price/unit (before TAX) |
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* Y: R, A, B or F plate type, according to the qPCR instrument
(see Compatibility file)*2: according to the number of ordered Microbiota SignArrays®
example: PZxHMB2-R12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument
** Please request for an estimate at: enquirie@anygenes.com
NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.
PMS Reagents associated with microbiota individual assays prices
Reference type : PMS1-Z100 | |
Perfect Master Mix SYBR® Green reagent (PMS) |
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Number of Reactions |
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* 10 μl of PMS/reaction for 20 μl final volume
(see Compatibility file)* Z: W, R, LR or F PMS, according to the qPCR instrument
* required number of reactions
Example: PMS1-W100 for Perfect Master Mix SYBR® Green for 100 reactions with the Light Cycler® 480 instrument
PMS Reagents associated with SignArrays® 96
prices
Reference type : PMS1-Z2S | |
PMS Perfect Master Mix SYBR® Green SignArrays® 96* |
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Number of SignArrays® 96 |
Price/unit (before TAX) |
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* 10 μl of PMS/reaction for 20 μl final volume
(see Compatibility file)* Z: W, R, LR or F PMS, according to the qPCR instrument
*2: according to the number of ordered Microbiota SignArray®
example: PMS1-W12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument
PMS Reagents associated with SignArrays® 384
prices
Reference type : PMS2-Z2S | |
PMS (Perfect Master Mix SYBR® Green) SignArrays® 384* |
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Number of SignArrays® 384 |
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* 5 μl of PMS/reaction for 10 μl final volumes
* Z: W, R, LR or F PMS, according to the qPCR instrument
(see Compatibility file)*2: according to the number of ordered Microbiota SignArrays®
** Please request for an estimate at: enquirie@anygenes.com
example: PMS2-W12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument