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LncRNA qPCR assays are essential tools for accurate quantification of long non-coding RNA expression, a critical step in gene expression studies and RNA-seq validation.
However, lncRNA analysis is technically challenging due to low expression levels, transcript complexity, and specificity issues.
Developed and experimentally validated by AnyGenes, a biotechnology company specialized in gene expression analysis and qPCR solutions, with a dedicated R&D team based in France and extensive experience in complex RNA analysis, these assays are designed to deliver reliable and reproducible results, even for complex lncRNA targets.
In addition to qPCR assays, AnyGenes provides gene expression analysis and RNA-seq validation services, supporting researchers worldwide.
LncRNAs present unique analytical difficulties compared to protein-coding genes:
These challenges often result in poor reproducibility and unreliable quantification when using non-validated primers.
Reliable quantification requires careful primer design and experimental validation.
Low-expression lncRNAs can be difficult to detect with standard qPCR workflows, especially when working with limited RNA input, FFPE samples, or single-cell material.
To improve detection sensitivity, cDNA preamplification can be applied prior to qPCR analysis. This strategy is particularly useful for lncRNA studies, where weak signals can limit accurate quantification.
The SpeAmp® qPCR preamplification system, developed by AnyGenes, is designed to enable reliable detection of low-abundance RNA targets while maintaining reproducible results.
This approach helps researchers:
These kits are designed to ensure high performance and reliability across a wide range of experimental conditions, including challenging samples.
They are developed with several key characteristics that support accurate and reproducible gene expression analysis:
Together, these features enable accurate detection and quantification of low-expressed and structurally complex lncRNAs.
These kits are available in flexible formats to suit different experimental needs:
Adapted to your biological model and research objectives
Selecting appropriate lncRNA qPCR assays depends on:
Validated assays ensure specificity, reproducibility, and reliable quantification, especially for challenging lncRNA targets.
AnyGenes products and services are available worldwide through a network of international distributors, supporting researchers across Europe, Asia, and North America.
These qPCR solutions are widely used in:
They are particularly valuable for:
LncRNA qPCR analysis often fails due to poor specificity and low signal detection.
For complex targets, experimentally validated assays are essential to ensure reproducibility and reliable quantification.
They are used to quantify lncRNA expression levels and validate gene expression data, particularly after RNA-seq experiments.
Because of low expression, isoform diversity, and potential overlap with coding genes, which can lead to non-specific amplification.
By using validated primers, targeting exon junctions, and optimizing assay conditions.
Yes. qPCR validation is essential to confirm RNA-seq results and ensure accurate expression analysis.
Whether you are validating RNA-seq results, working with low-expression targets, or exploring new biomarkers, AnyGenes provides reliable and experimentally validated lncRNA qPCR assays and dedicated scientific support tailored to your research needs..
Contact our team to discuss your project.