HEMATOPOIETIC STEM CELLS: THE FOUNDATION OF BLOOD CELL DEVELOPMENT

Hematopoietic stem cells (HSCs) are multipotent stem cells located primarily in the bone marrow, responsible for the generation of all blood cell lineages. HSCs possess the unique ability to self-renew and differentiate into various blood cell types. They serve as the foundation for therapies targeting hematologic diseases, genetic disorders, and cancer treatments.

At AnyGenes, we offer specialized qPCR arrays designed for the analysis of key genes involved in HSCs biology. Our tools support researchers in uncovering the complexities of HSCs and their potential applications in regenerative medicine and cancer therapy.

Bone marrow microenvironment with their components.

CHARACTERISTICS OF HSCs

HSCs possess unique characteristics that distinguish them from other stem cell types:

Multipotency: HSCs can differentiate into all mature blood cell types, including red blood cells, white blood cells, and platelets.
Self-Renewal: HSCs can replicate themselves indefinitely, ensuring a continuous supply of stem cells.
Niche Dependency: HSC function is heavily influenced by their microenvironment, known as the stem cell niche, which comprises various supporting cells and extracellular matrix components

HEMATOPOIETIC STEM CELLS LINEAGES & NICHE

Myeloid Lineage: This lineage produces erythrocytes (red blood cells), platelets, and various types of white blood cells (e.g., monocytes, granulocytes).
Lymphoid Lineage: This lineage generates lymphocytes, including B cells and T cells, which are critical for the immune response.

The bone marrow is the primary site for HSCs, providing a specialized microenvironment that regulates their behavior:

Extracellular Matrix (ECM): The ECM consists of proteins like fibronectin and collagen that facilitate HSC attachment and signaling necessary for proliferation and differentiation.
Stromal Cells: These cells secrete essential growth factors such as CXCL12 and SCF, crucial for HSC maintenance and proliferation.
Mechanical Cues: The stiffness of the bone marrow niche influences HSC behavior, guiding their motility and differentiation potential.

HSCs BIOMARKERS: KEY TOLLS FOR STEM CELL RESEARCH

HSCs are characterized by specific surface markers that facilitate their identification and isolation. These biomarkers play a crucial role in both research and clinical applications, including transplantation and disease monitoring.

CD34: A primary marker used to identify human HSCs, typically expressed at high levels in stem cells but decreases as cells differentiate.
CD38 Generally low or absent in HSCs, this marker helps differentiate stem cells from more mature progenitor cells.
c-Kit (CD117): This receptor for stem cell factor is crucial for HSC survival and proliferation, often expressed on HSCs.
Sca-1: A marker commonly used in mouse models, indicating stem cell properties. CD90 (Thy-1): Expressed on HSCs and plays a role in cell adhesion and signaling. CD133: important marker for human HSCs, associated with the ability to repopulate the hematopoietic system effectively. CD150: A marker associated with long-term repopulating HSCs, indicating their potential for self-renewal.

THERAPEUTICS APPLICATIONS

HSCs are crucial for various therapeutic applications:

Bone Marrow Transplantation: HSCs are commonly used in the treatment of hematological malignancies (e.g., leukemia) and other disorders by restoring healthy blood cell production.
Gene Therapy: Researchers are exploring the use of HSCs for delivering gene therapies, particularly for genetic blood disorders.
Regenerative Medicine: HSCs hold promise for treating conditions related to blood cell deficiencies and autoimmune diseases.
Disease Monitoring: Changes in the expression of HSCs biomarkers can indicate disease states such as infections (like COVID-19), where HSC populations may be altered significantly.

(1) Kwon M, et al. Hematopoietic Stem Cells and Their Niche in Bone Marrow. Int J Mol Sci. (2024)21;25(13):6837.

(2) Hussein HAM, et al. Phenotypical changes of hematopoietic stem and progenitor cells in COVID-19 patients: Correlation with disease status. Cent Eur J Immunol. (2023);48(2):97-110.

(3) Yadav P, et al. Hematopoietic Stem Cells Culture, Expansion and Differentiation: An Insight into Variable and Available Media. Int J Stem Cells. (2020)31;13(3):326–334.

HEMATOPOIETIC STEM CELLS BIOMARKER LIST

Customize your own signaling pathways (SignArrays®) with the factors of your choice!
Simply download and complete our Personalized SignArrays® information file and send it at [email protected] to get started on your project.

You can check the biomarker list included in this pathway, see below:

Species : Homo sapiens (human)

Signaling Pathways	Product ref	Informations
Hematopoietic Stem Cells	HSC1H1	List infos
Hematopoietic Stem Cells	HSC2H1	List infos
Hematopoietic Stem Cells	HSC3H1	List infos
Hematopoietic Stem Cells	HSC4H1	List infos

Species : Mus musculus (mouse)

Signaling Pathways	Product ref	Informations
Hematopoietic Stem Cells	*******	List In progress...

Species : Rattus norvegicus (rat)

Signaling Pathways	Product ref	Informations
Hematopoietic Stem Cells	*******	List In progress...

Species : Sus Scrofa (Pig)

Signaling Pathways	Product ref	Informations
Hematopoietic Stem Cells	*******	List In progress...

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