Validated Primer Sets title   Identification of Mycoplasma contamination by fast and highly efficient qPCR assays

Cell culture contamination by Mycoplasma is a real problem in laboratories. These small bacteria, invisible at light microscopes, drastically alter the cells, with serious consequences on cell metabolism (cellular death, impaired growth, absence of transfection, morphological and phenotype modifications, impaired gene expression profiles...) and hence on the reliability of the data obtained with using contaminated cultures. Recently, it has been reported that approximately 15–35% of continuous cell lines are contaminated by Mycoplasmas [1].

MycoDiag assays ensure a fast and reliable screening of your cell culture supernatants for Mycoplasma contamination by qPCR or PCR .


In order to identify potential Mycoplasma contamination in your cell culture, AnyGenes developed the MycoDiag assay. It allows quick and efficient detection of specific Mycoplasma DNA, directly from cell culture supernatants. The analysis is based on Real-Time qPCR or Endpoint PCR, following your platform.


Based on specific primer sets designed with very stringent criteria and experimentally validated by strict quality control process, the performance of AnyGenes® MycoDiag assay has been carefully optimized to provide a very high sensitivity and specificity. MycoDiag assay can detect the 25 most representative Mycoplasma species found in contaminated cell culture [2] (see list below).


M. agalactiae,
M. genitalium,
M. pulmonis,
M. arginini,
M. hominis,
M. salivarium,
M. arthritidis,
M. hyorhinis,
M. spermatophilum,
M. bovis,
M. hyosynoviae,
M. synoviae,
M. cloacale,
M. opalescens,
M. timone,
M. falconis,
M. orale,
A. laidlawii,
M. faucium,
M. pirum,
Spiroplasma,
M. fermentans,
M. pneumoniae M129,
U. urealyticum.
M. gallisepticum,

MycoDiag assays ensure a fast and reliable screening of your cell culture supernatants in less than 2 hours with your qPCR instrument, supported by efficient quality controls (Negative Control, Positive Control with a Mycoplasma DNA, and Internal Control to validate the absence of PCR inhibitors in your samples) which avoid false negative results.


With highly sensitive and specific primer sets, MycoDiag assays ensure the detection of Mycoplasma DNA in your samples by qPCR / PCR.
Agarose gel electrophoresis, after PCR with MycoDiag assays, allows to observe bands between 121 to 230 bp, in case of Mycoplasma contamination of cell culture supernatants.
Legend: Samples 1, 2 and 3 : negative results ; Sample 4 : positive result, so Mycoplasma contamination in this cell culture supernatant ; POS CTR : Positive Control with bands at 129-142 and 230 bp, NEG CTR : Negative Control , absence of amplification

MycoDiag assay is now used as the tool of choice in many research laboratories, in public and private sector, to enable routine checks of cell cultures.

Validated Primer Sets title   The advantages of MycoDiag assay

Compatible with all the qPCR / PCR instruments, these highly specific and sensitive easy-to-use MycoDiag assays allow to detect Mycoplasma contamination of your cell cultures in less than 2 hours.

MycoDiag assays prices

Catalog Reference Number of
Reactions
Price
(before TAX)
MycoDiag-X20
20
*
MycoDiag-X50
50
*
MycoDiag-X100
100
*

X : W, R, LR, F, according to the qPCR instrument or P for EndPoint PCR application (Please see our compatibility file)


Download our MycoDiag Assay procedures for more information. Click here .

** For more information on prices, please contact us at [email protected]

*** These prices are available only in FRANCE, please contact your local distributors of your country


(1) Tantibhedhyangkul W et al. Anti-Mycoplasma Activity of Daptomycin and Its Use for Mycoplasma Elimination in Cell Cultures of Rickettsiae. Antibiotics (Basel). (2019); 8(3): 123

(2) McGarrity G. et al. Mycoplasmas and tissue culture cells. (1992). In: Maniloff, J., McElhaney, R.N., Finch, L.R., Baseman, J.B. (Eds.), Mycoplasmas, Molecular Biology and Pathogenesis, American Society for Microbiology, 445-54