What is the PI3K/AKT signaling pathway gene?

The pathway involves multiple genes: PIK3CA, AKT1/2/3, PTEN, and downstream effectors like mTOR. Studying these genes clarifies the pathway’s role in health and disease.

What is the PI3K/AKT NF-κB signaling pathway?

PI3K/AKT interacts with NF-κB, enhancing its activity to promote cell survival, inflammation, and therapy resistance.

What is the PI3K/AKT signaling pathway for apoptosis?

Activated AKT phosphorylates pro-apoptotic proteins like BAD and caspase-9, while increasing survival proteins such as Bcl-2, preventing programmed cell death.

How can I study PI3K/AKT biomarkers with AnyGenes® qPCR arrays?

You can explore our curated list of PI3K/AKT biomarkers in the table below to check if they align with your research objectives. If your genes of interest are not listed,you can customize your own SignArrays® by downloading and completing our Personalized SignArrays® information file. Send it to contact@anygenes.com to get started on your project.

PI3K/AKT Signaling Pathway – Functions, Disease Implications and Biomarker Analysis

Q: How does the PI3K/AKT pathway work?

The pathway is activated by growth factors binding to RTKs, PI3K produces PIP3, and AKT phosphorylates downstream proteins to regulate cell survival, growth, metabolism, and angiogenesis.

What is the PI3K/AKT signaling pathway?

The PI3K/Akt signaling pathway is a central intracellular signaling cascade that regulates proliferation, angiogenesis, cell growth, survival, metabolism, and motility. It is activated by extracellular signals such as growth factors, cytokines, hormones, and nutrients.

Upon activation, phosphoinositide 3-kinase (PI3K) converts PIP2 into PIP3, leading to recruitment and activation of AKT (Protein Kinase B). Activated AKT phosphorylates a wide range of downstream substrates controlling cell fate decisions.

Dysregulation of PI3K/AKT signaling is one of the most frequent molecular alterations in cancer, and is also implicated in metabolic, cardiovascular, and neurodegenerative diseases.

PI3K/AKT signaling pathway biomarker list
View the genes included in our PI3K/AKT pathway qPCR arrays.

Upstream activation of the PI3K/Akt signaling pathway.

Key takeaways

Master regulator of cell survival and growth
Activated downstream of growth factor receptors
Strongly linked to cancer progression and therapy resistance
Interacts with mTOR, NF-κB, MAPK, and apoptosis pathways
Ideal for biomarker discovery and gene expression profiling

Core components of the PI3K/AKT signaling pathway

PI3K

Catalyzes the production of PIP3, initiating AKT activation.

AKT (Protein Kinase B)

Central kinase regulating metabolism, proliferation, and anti-apoptotic signaling.

mTOR

Downstream effector controlling protein synthesis, cell growth, and metabolism.

PTEN

Tumor suppressor that negatively regulates PI3K signaling by dephosphorylating PIP3.

Biological functions of PI3K/AKT signaling

Cell growth and proliferation
Cell survival and apoptosis inhibition
Metabolic regulation (glucose and lipid metabolism)
Angiogenesis and vascular remodeling
Cell migration and invasion

PI3K/AKT signaling pathway in disease

Cancer: Hyperactivation promotes:

Uncontrolled proliferation
Resistance to apoptosis
Metastasis
Resistance to targeted therapies

Mutations in PIK3CA, AKT, or loss of PTEN are common across solid tumors.

Metabolic diseases: Altered signaling contributes to:

Insulin resistance
Type 2 diabetes
Obesity

Neurodegenerative and cardiovascular diseases: PI3K/AKT dysregulation affects neuronal survival and cardiac function

Cross-talk with other signaling pathways

PI3K/AKT signaling interacts extensively with:

This network integration makes PI3K/AKT a central hub in cellular signaling

Therapeutic targeting of PI3K/AKT signaling

Because of its central role, PI3K/AKT is a major therapeutic target:

PI3K inhibitors
AKT inhibitors
Dual PI3K/mTOR inhibitors

Accurate biomarker profiling is essential to guide therapeutic strategies

Why study the PI3K/AKT signaling pathway with AnyGenes

At AnyGenes®, we provide high-performance qPCR arrays and customizable SignArrays® for PI3K/AKT pathway analysis and biomarker discovery.

Our solutions allow researchers to:

Quantify PI3K/AKT-dependent gene signatures
Analyze downstream and cross-talk pathways
Identify predictive and prognostic biomarkers
Generate robust, publication-ready data

PI3K/AKT signaling pathway biomarker analysis with AnyGenes®

What can be analyzed?

Core pathway kinases and regulators
Downstream transcriptional targets
Apoptosis- and metabolism-related genes
Cancer-associated PI3K/AKT biomarkers

Customize your own signaling pathways (SignArrays^®) with the factors of your choice!
Simply download and complete our Personalized SignArrays^® information file and send it at [email protected] to get started on your project.

Frequently asked questions

How does the PI3K/AKT pathway work?

It is activated by growth factor receptors, leading to PI3K-mediated PIP3 production and AKT activation, which regulates cell survival, growth, and metabolism

Which genes are involved in the PI3K/AKT signaling pathway?

Key genes include PIK3CA, AKT1/2/3, PTEN, and mTOR.

How is PI3K/AKT signaling involved in cancer?

Hyperactivation promotes tumor growth, survival, and resistance to therapy.

How can PI3K/AKT pathway activity be analyzed?

By measuring expression of pathway regulators and downstream targets using targeted gene expression approaches such as qPCR pathway arrays

Jafari M et al. PI3k/AKT signaling pathway: Erythropoiesis and beyond. J Cell Physiol. (2019);234(3):2373-2385.
He Y et al. Targeting PI3K/Akt signal transduction for cancer therapy. Signal Transduct Target Ther. (2021);6(1):425.
Liu R et al. PI3K/AKT pathway as a key link modulates the multidrug resistance of cancers. Cell Death Dis. (2020);11(9):797.
Teng Y et al. The PI3K/Akt Pathway: Emerging Roles in Skin Homeostasis and a Group of Non-Malignant Skin Disorders. Cells. (2021);10(5):1219.
Chu E et al. Dysregulated phosphoinositide 3-kinase signaling in microglia: shaping chronic neuroinflammation. J Neuroinflammation. (2021);18(1):276.

PI3K-AKT signaling pathway biomarker list

You can check the biomarker list included in this pathway, see below:

Species : Homo sapiens (human)

Human Signaling Pathways

Signaling Pathways	Product ref	Informations
PI3K-AKT Signaling Pathway	AKT1H1	List infos

Species : Mus musculus (mouse)

Mouse Signaling Pathways

Signaling Pathways	Product ref	Informations
PI3K-AKT Signaling Pathway	AKT1M1	List infos

Species : Rattus norvegicus (rat)

Rat Signaling Pathways

Signaling Pathways	Product ref	Informations
PI3K-AKT Signaling Pathway	*******	List In progress...

Species : Sus Scrofa (Pig)

Pig Signaling Pathways

Signaling Pathways	Product ref	Informations
PI3K-AKT Signaling Pathway	*******	List In progress...

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