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AnyGenes® provides qPCR-based solutions for single cell analysis, combining direct lysis, specific preamplification and SignArrays® qPCR arrays to support targeted gene expression profiling from very limited biological material.
Designed for researchers working with rare cells, low-input samples or selected cell populations, this workflow helps analyze genes, biomarkers and signaling pathways using a streamlined qPCR approach.
AnyGenes® single cell qPCR solutions are designed for projects requiring targeted gene expression analysis from individual cells, rare cell populations or low-input biological samples.
Supports gene expression analysis from very limited biological material, including rare or sorted cells.
Helps simplify sample preparation while reducing RNA loss associated with conventional extraction steps.
Supports targeted amplification of selected cDNA targets before qPCR analysis.
Enables targeted analysis of selected signaling pathways and biomarker panels by qPCR.
Designed for targeted quantification of selected genes, transcripts or pathway signatures.
Can be combined with AnyGenes® validated primers, Master Mixes and data analysis tools.
Single cell analysis by qPCR requires careful control of each experimental step, from cell collection and lysis to reverse transcription, preamplification, qPCR and data interpretation.
AnyGenes® combines DiLysis™, SpeAmp® and SignArrays® to support a targeted workflow for gene expression profiling when starting material is limited.
DiLysis™ helps prepare cell lysates while limiting sample handling and potential RNA loss.
SpeAmp® supports specific preamplification of selected targets before qPCR analysis.
SignArrays® and validated qPCR assays can be used to profile selected genes and signaling pathways.
Combine direct lysis, specific preamplification and SignArrays® qPCR arrays to support targeted gene expression analysis from rare cells, low-input RNA samples and limited biological material.
SpeAmp® reagents support efficient and specific preamplification for rare samples, low-input RNA workflows and targeted gene expression analysis before qPCR.
DiLysis™ reagents support direct lysis workflows for single cell analysis and low-input qPCR, helping reduce sample handling and potential RNA loss before downstream gene expression analysis.
AnyGenes® qPCR solutions can support targeted analysis of rare cells, low-input samples and selected biological pathways.
Analyze gene expression in limited cell populations obtained by sorting, isolation or enrichment.
Explore selected gene expression differences between individual cells or defined cell groups.
Study selected immune markers, cytokines or pathway genes in low-input immune cell samples.
Support targeted analysis of tumor cells, rare cell populations or selected biomarker candidates.
Analyze selected genes involved in cell identity, differentiation or developmental pathways.
Validate selected transcriptomic findings using targeted qPCR assays when starting material is limited.
Single cell analysis by qPCR can be strengthened by combining direct lysis, preamplification, validated qPCR primers, Master Mixes and SignArrays® pathway panels within a coherent workflow.
Use experimentally validated primer sets to analyze selected gene targets by real-time PCR.
Combine single cell workflows with compatible SYBR® Green or probe-based qPCR reagents.
Profile selected signaling pathways or build personalized panels using your genes of interest.
AnyGenes® combines expertise in qPCR, gene expression analysis, preamplification and pathway-focused profiling to support targeted single cell and low-input workflows.
Support for experimental strategy, assay selection and targeted gene expression analysis.
DiLysis™ and SpeAmp® are designed for workflows involving limited biological material.
SignArrays® panels can help analyze selected signaling pathways and biomarker signatures.
Combine lysis, preamplification, validated primers, qPCR reagents and data analysis tools.
Personalized SignArrays® can be designed according to your selected genes and research objectives.
AnyGenes® can help adapt the workflow to your sample type, input level and target genes.
AnyGenes® reagents and related technologies have been referenced in peer-reviewed scientific publications, including journals such as Nature, Circulation and PLOS ONE.
These publications illustrate the use of AnyGenes® technologies in molecular biology, gene expression analysis and biomarker-focused research workflows.
AnyGenes® can help you select the appropriate direct lysis, preamplification, qPCR assay and SignArrays® workflow according to your sample type, target genes and research objective.
Whether your project involves rare cells, low-input samples, pathway profiling, biomarker validation or RNA-seq follow-up, our team can help define a targeted qPCR strategy.
Single cell analysis by qPCR is a targeted approach used to quantify selected gene expression markers from individual cells or very small cell populations using real-time PCR.
Direct lysis can reduce sample handling and help limit RNA loss, which is important when working with individual cells, rare cell populations or low-input samples.
Preamplification increases the amount of selected cDNA targets before qPCR, which can help analyze weakly expressed genes or limited input material.
Yes. Single cell qPCR workflows can be combined with selected SignArrays® panels to profile genes involved in signaling pathways or biomarker-focused research.
Yes. AnyGenes® single cell workflows can be combined with validated qPCR primers, qPCR Master Mixes and SignArrays® panels for targeted gene expression analysis.
Yes. AnyGenes® can help select direct lysis, preamplification, qPCR assays and SignArrays® panels according to your sample type, input level and selected genes.
Visit the AnyGenes® Help & FAQ section for detailed information about our products, services, ordering process and technical support.
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