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AnyGenes® Perfect Master Mix Probe is a ready-to-use Probe qPCR Master Mix designed for real-time PCR workflows using hydrolysis probes, including TaqMan®-style assays and multiplex qPCR applications.
Developed for molecular biology laboratories working on gene expression analysis, biomarker validation, SNP genotyping, CNV analysis, pathogen detection and targeted molecular quantification, this 2X probe-based qPCR reagent helps simplify reaction setup while supporting reproducible amplification.
Perfect Master Mix Probe is designed for researchers who need a ready-to-use qPCR reagent for probe-based real-time PCR workflows requiring specificity, efficiency and reproducibility.
Designed to simplify probe-based qPCR reaction setup and reduce preparation time.
Suitable for hydrolysis probe workflows, including TaqMan®-style real-time PCR assays.
Formulated with Hot Start polymerase to support specific and efficient amplification.
Can support multiplex probe qPCR when assays, fluorophores and reaction conditions are properly designed.
Available with ROX, Low ROX, fluorescein or dye-free formats according to instrument requirements.
Supports gene expression, SNP genotyping, CNV, pathogen detection and other targeted qPCR workflows.
Perfect Master Mix Probe combines probe-based detection chemistry, optimized buffer components and a Hot Start Taq polymerase to support efficient amplification in real-time PCR assays.
This formulation is designed to support specific fluorescence detection in hydrolysis probe assays, helping reduce non-specific signal when primers, probes and reaction conditions are properly designed.
The reagent can be used in targeted qPCR workflows involving gene expression analysis, multiplex probe qPCR, SNP genotyping, copy number variation analysis and molecular detection.
This probe-based formulation is developed to support reliable target quantification across a range of real-time PCR research applications.
Supports target-specific detection using hydrolysis probes in real-time PCR workflows.
Formulated to support efficient amplification in validated probe-based qPCR assays.
Can be used in multiplex qPCR workflows when assay design and fluorophore selection are appropriate.
Available in configurations adapted to common real-time PCR instrument requirements.
Suitable for gene expression, genotyping, CNV, pathogen detection and biomarker research.
Supports consistent amplification performance for molecular biology and qPCR research projects.
Perfect Master Mix Probe can support multiple molecular biology applications where specific probe-based detection is required.
Support simultaneous detection of selected targets using compatible probes and fluorophores.
Quantify selected genes, transcripts or expression signatures using fluorescent probe assays.
Support single-nucleotide polymorphism analysis using allele-specific probe-based assays.
Support targeted detection of DNA regions in chromatin immunoprecipitation workflows.
Support copy number variation analysis using quantitative real-time PCR.
Measure selected transcript isoforms using carefully designed probe-based assays.
Use probe-based qPCR workflows for targeted microbial or pathogen detection.
Support targeted quantification of selected microbial communities or species.
Support quantitative monitoring of selected viral targets in research workflows.
Some probe-based qPCR projects involve challenging samples or target regions, including FFPE-derived nucleic acids, laser capture microdissection samples, low-input material or GC-rich target regions.
Perfect Master Mix Probe is designed to support reliable amplification in probe-based real-time PCR workflows. For demanding samples, assay design, nucleic acid quality, primer/probe specificity and workflow optimization remain important for reproducible results.
Can support targeted qPCR workflows using fragmented or limited nucleic acids when assays are properly designed and sample quality is assessed.
Suitable for workflows involving limited biological material, especially when combined with appropriate sample preparation or pre-amplification strategies.
Can be used for selected GC-rich targets, while primer/probe design and cycling conditions remain critical for amplification specificity.
Perfect Master Mix Probe is available in different reference dye configurations to match common qPCR instrument requirements.
For instruments requiring passive ROX reference dye.
For qPCR platforms requiring lower passive reference dye concentration.
For systems requiring fluorescein or workflows without passive reference dye.
Perfect Master Mix Probe is available in multiple reaction sizes to support routine, medium-throughput and larger probe-based qPCR projects.
Reference type: PMP1-Z 50
Z corresponds to the selected reference dye configuration: W, R, LR or F, according to qPCR instrument compatibility.
Recommended use: 10 µl of PMP per reaction for a 20 µl final reaction volume.
Select the appropriate reaction number according to project size and experimental design.
| Reference type : PMP1-Z50 | |
| Perfect Master Mix PROBE | |
| Number of Reactions | Price/unit (before TAX) |
|---|---|
* 10 μl of PMP/reaction for 20 μl final volume
* Z : W, R, LR or F Perfect Master Mix PROBE, according to the qPCR instrument
AnyGenes® develops qPCR reagents, validated primer sets and qPCR arrays for researchers working on gene expression analysis, signaling pathways and biomarker-focused projects.
Developed by a team experienced in qPCR workflows, gene expression analysis and molecular assay design.
Compatible with AnyGenes® validated primers, SignArrays® and targeted biomarker workflows.
Available in reference dye configurations adapted to common real-time PCR platforms.
Suitable for workflows involving multiplex qPCR, genotyping, CNV, ChIP-qPCR or selected low-input projects.
Support for choosing the appropriate format, compatible workflows and qPCR experimental strategy.
AnyGenes® technologies have been referenced in peer-reviewed publications across molecular biology research fields.
AnyGenes® reagents and related technologies have been referenced in peer-reviewed scientific publications, including journals such as Nature, Circulation and PLOS ONE.
These publications illustrate the use of AnyGenes® technologies in molecular biology, gene expression analysis and biomarker-focused research workflows.
AnyGenes® can help you select the appropriate reference dye configuration, evaluate your probe-based qPCR workflow and choose complementary solutions such as validated primers, SignArrays® or qPCR arrays.
Whether your project involves multiplex qPCR, gene expression analysis, genotyping, CNV, biomarker validation or targeted molecular detection, our team can help you identify the most suitable format.
A Probe qPCR Master Mix is a ready-to-use reagent for real-time PCR workflows using fluorescent probes. It can support gene expression analysis, multiplex qPCR, SNP genotyping, CNV analysis, pathogen detection and other targeted molecular biology applications.
Probe-based qPCR uses target-specific fluorescent probes for detection, while SYBR® Green qPCR detects amplified double-stranded DNA using a fluorescent dye. Probe-based workflows can provide higher target specificity when assays are properly designed.
Yes. It can support multiplex probe qPCR workflows when primers, probes, fluorophores and reaction conditions are carefully designed and validated.
It is available with different reference dye configurations, including ROX, Low ROX, fluorescein or dye-free options. The correct format should be selected according to your instrument compatibility file.
The reagent is designed to simplify probe-based qPCR setup. However, primer and probe design, sample quality, fluorophore compatibility and instrument settings remain important for reliable results.
You can contact the AnyGenes® team to request pricing, product information, compatibility guidance or support for your probe-based qPCR project.
Visit the AnyGenes® Help & FAQ section for detailed information about our products, services, ordering process and technical support.
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