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AnyGenes® SpeAmp® is a qPCR preamplification kit designed to support targeted cDNA preamplification before real-time PCR, qPCR array analysis and pathway-focused gene expression profiling.
The workflow is particularly useful when RNA input is limited, target abundance is low, or biological material is difficult to obtain, including single-cell samples, FFPE-derived RNA, laser capture microdissection samples and rare cell populations.
SpeAmp® supports targeted preamplification of selected cDNA targets before qPCR analysis, helping researchers work with limited RNA quantities and weakly expressed genes.
Designed for workflows starting from limited RNA quantities, including applications using as little as 5 ng of RNA depending on sample quality and experimental design.
Amplifies selected cDNA targets before qPCR rather than performing whole-transcriptome amplification.
Can support the analysis of selected SignArrays® pathway panels and biomarker-focused gene sets.
Supports large targeted gene panels, including workflows covering up to 368 genes.
Can be used for profiling up to 4 signaling pathways in adapted SignArrays® workflows.
Suitable for low-input RNA, FFPE-derived RNA, LCM samples, PBMCs, rare cells and RNA-seq validation.
Low-input transcriptomic experiments often generate weak amplification signals, increased Cq variability and inconsistent detection of low-abundance transcripts. These issues are common when working with rare samples, single-cell material, FFPE-derived RNA, small biopsies or laser capture microdissection samples.
SpeAmp® supports qPCR preamplification by increasing the amount of selected cDNA targets before real-time PCR analysis, while keeping the workflow focused on selected genes, signaling pathways or biomarker panels.
RNA is converted into cDNA using an adapted reverse transcription workflow.
Selected cDNA targets are preamplified using SpeAmp® and appropriate pooled primers.
Preamplified cDNA is analyzed using qPCR assays, SignArrays® panels or selected gene expression workflows.
SpeAmp® has been developed by AnyGenes® to support reproducible cDNA preamplification before qPCR array analysis.
Validated experiments demonstrated strong correlation between preamplified and non-preamplified samples, supporting reliable biological interpretation after cDNA preamplification.
Key validation results
Independent experiments supported consistent amplification performance for pathway-focused
qPCR analysis and low-input transcriptomic workflows.
SpeAmp® can be combined with AnyGenes® SignArrays® pathway panels, Perfect Master Mix SYBR® Green and Primnx pooled primer systems to build targeted qPCR preamplification workflows.
Supports pathway-focused qPCR array workflows for selected signaling pathways, biological processes and biomarker panels.
Primer pools can be adapted to selected genes, SignArrays® panels, custom transcriptomic signatures or specific biological pathways.
Can be used within compatible SYBR® Green-based real-time PCR workflows for downstream gene expression analysis.
SpeAmp® is designed for molecular biology projects where target abundance, RNA quantity or sample availability can limit direct qPCR analysis.
Supports targeted qPCR analysis when working with individual cells or very limited cellular material.
Helps increase selected cDNA target quantity before qPCR when RNA input is limited.
Can support qPCR workflows involving fragmented or challenging RNA from archived samples.
Useful for projects involving laser capture microdissection or limited tissue material.
Supports targeted validation of selected RNA-seq findings by qPCR when starting material is limited.
Can be combined with SignArrays® panels to analyze selected pathways or biomarker signatures.
The SpeAmp® system includes reagents for targeted cDNA preamplification and can be combined with optional pooled primers according to the selected qPCR workflow.
Optimized amplification mix containing thermostable Taq DNA polymerase, amplification buffer, MgCl₂ and dNTPs for cDNA preamplification.
Enzymatic purification system designed to clean preamplification products before downstream qPCR analysis.
Optional pooled primers corresponding to selected SignArrays® pathway panels, custom gene signatures or specific biological pathways.
SpeAmp® kits and Primnx pooled primers are available for different reaction formats. Contact AnyGenes® for quotations, product information or distributor details.
| Catalog Reference | Number of Reactions | Price |
|---|---|---|
XXXX : name of the selected specific signaling pathway
example : AN1H for human angiogenesis 1
| Catalog Reference | Number of Reactions | Price |
|---|---|---|
SpeAmp® can be integrated with other AnyGenes® qPCR solutions to support single-cell, low-input and pathway-focused gene expression projects.
Combine direct lysis, preamplification and SignArrays® panels for targeted single-cell qPCR workflows.
Use DiLysis™ to support direct lysis workflows for rare cells and limited biological material.
Use validated primer sets for targeted qPCR analysis of selected genes and biomarkers.
AnyGenes® can help you define the most appropriate qPCR preamplification strategy according to your sample type, RNA input, target genes, SignArrays® panel and downstream analysis.
Whether your project involves single-cell analysis, FFPE-derived RNA, LCM samples, RNA-seq validation, lncRNA profiling or pathway-focused qPCR, our team can help you build a targeted workflow.
A qPCR preamplification kit amplifies selected cDNA targets before real-time PCR analysis to support detection of low-abundance transcripts or workflows starting from limited RNA.
qPCR preamplification is useful when RNA input is limited, target genes are weakly expressed, or samples are difficult to obtain, such as single-cell material, FFPE-derived RNA, LCM samples, rare cells or small biopsies.
Yes. SpeAmp® can be combined with AnyGenes® SignArrays® pathway panels and Primnx pooled primers for pathway-focused qPCR preamplification workflows.
SpeAmp® can support workflows starting from limited RNA quantities, including applications using as little as 5 ng of RNA, depending on sample quality, target abundance and experimental design.
Yes. SpeAmp® can support targeted qPCR validation of selected RNA-seq results, especially when remaining RNA material is limited.
Yes. Primnx pooled primers can be adapted to selected genes, biomarker panels, signaling pathways or custom transcriptomic signatures.
Visit the AnyGenes® Help & FAQ section for detailed information about our products, services, ordering process and technical support.
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