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AnyGenes® Microbiota qPCR assays are designed to support targeted microbiome profiling by real-time PCR, using experimentally evaluated primer sets and flexible qPCR formats for microbial strain detection and microbiota research.
Available as individual microbiota assays or customizable Microbiota SignArrays®, these solutions help researchers analyze selected microbial species, compare sample groups and build targeted microbiota panels according to their research objectives.
AnyGenes® ready-to-use Microbiota assays are designed for targeted qPCR analysis of selected microbial species and strains, with flexible formats for individual targets or larger customized panels.
Analyze selected microbial species or strains using qPCR-based assays adapted to defined microbiota research questions.
Microbiota primer sets are evaluated using microbial DNA collections and qPCR quality controls.
After qPCR validation, selected amplicons can be sequenced to support assay specificity assessment.
Choose individual microbiota assays or build customized Microbiota SignArrays® in 96- or 384-well formats.
Assays can include controls to help assess assay performance, sample quality and possible DNA contamination.
Designed for use with AnyGenes® Perfect Master Mix SYBR® Green and compatible qPCR instruments.
Microbiota qPCR assays provide a targeted approach for studying selected microbial species, strains or panels. Unlike broad exploratory methods, targeted qPCR focuses on predefined microbial targets that are relevant to a specific biological question.
AnyGenes® Microbiota qPCR assays can be used to follow microbial signatures across sample groups, validate selected microbiome findings, or build customized qPCR panels for focused microbiota studies.
Choose species, strains, body sites or biological themes according to your research objective.
Use individual microbiota assays or customizable Microbiota SignArrays® 96 / 384 formats.
Perform targeted qPCR analysis using compatible reagents, controls and data interpretation tools.
AnyGenes® proposes several microbiota qPCR formats to support projects ranging from individual microbial targets to larger customized microbiota panels.
Individual qPCR assays for selected microbial species or strains, suitable for focused detection or validation of specific microbiota targets.
Customizable 96-well qPCR panel format for targeted microbiota profiling across selected microbial targets.
Customizable 384-well qPCR panel format for larger targeted microbiota studies and higher-throughput projects.
AnyGenes® has developed a microbiota database based on microbial strains and published microbiota research to help researchers select relevant targets for Microbiota qPCR assays and customized Microbiota SignArrays®.
Researchers can search microbial targets by host species, taxonomy level or body site to build panels adapted to their experimental model and microbiota research question.
Select microbial targets relevant to human samples or laboratory models such as mouse or rat.
Explore targets by phylum, class, order, family, genus or species according to your project.
Build panels for microbiota studies focused on gut, oral cavity, skin or other sample contexts.
Microbiota qPCR assays can support targeted studies in microbiome research, host-microbe interactions and biomarker-oriented projects.
Analyze selected microbial species or strain signatures in gut microbiota research.
Support targeted qPCR analysis of microbial targets in skin-associated samples.
Study selected microbial species in oral cavity or salivary microbiota samples.
Explore selected microbiota signatures in projects related to immune regulation, inflammation or host response.
Support targeted microbiota analysis in research projects exploring microbiome-related tumor or tissue environments.
Build targeted panels for selected microbial targets involved in antibiotic resistance or microbial ecology research.
Microbiota qPCR assays require careful primer design, microbial target selection and experimental validation to support specificity and reliable interpretation.
AnyGenes® Microbiota assays are evaluated using microbial DNA collections and qPCR-based quality controls. Amplicon sequencing can be used after qPCR validation to support specificity assessment for selected microbial targets.
Assays are designed to target selected microbial species or strains while limiting non-specific amplification risks.
qPCR performance can be evaluated through amplification behavior, controls and assay-specific validation steps.
Controls can help assess assay performance, sample quality and possible DNA contamination.
AnyGenes® Microbiota assays are available as individual assays or customizable SignArrays® panels. Contact AnyGenes® for quotations, compatibility information and support with panel design.
| Reference type : MB–XXXX–100 | |
| Microbiota individual assays | |
| Number of Reactions | Price (before TAX) |
|---|---|
*XXXX = microbial species name (according to the NCBI Taxonomy Browser approved nomenclature)
* required number of reactions
* MB for Microbiota
Example: ME-B.vulgatus.8482-100 for the Bacteroides vulgatus ATCC 8482 species for 100 reactions
| Reference type : PZxHMB1-Y2S | |
| Customizable | |
| Number of SignArrays® 96 | Price/unit (before TAX) |
|---|---|
* Y: R, A, B or F plate type, according to the qPCR instrument (cf Compatibility file)
*2: according to the number of ordered Microbiota SignArrays®
example: PZxHMB1-R12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument
NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.
| Reference type : PZxHMB2-Y2S | |
| Customizable | |
| Number of SignArrays® 384 | Price/unit (before TAX) |
|---|---|
* Y: R, A, B or F plate type, according to the qPCR instrument
*2: according to the number of ordered Microbiota SignArrays®
example: PZxHMB2-R12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument
** Please request for an estimate at: [email protected]
NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.
AnyGenes® Microbiota assays are designed for use with compatible Perfect Master Mix SYBR® Green reagents according to assay format, reaction volume and qPCR instrument.
Perfect Master Mix SYBR® Green reagents can be provided for individual microbiota qPCR assays in selected reaction formats.
Associated PMS reagents can be selected according to SignArrays® 96 format and compatible qPCR instrument.
Associated PMS reagents are available for 384-well microbiota panel workflows and adapted reaction volumes.
| Reference type : PMS1-Z100 | |
| Perfect Master Mix SYBR® Green reagent (PMS) | |
| Number of Reactions | Price (before TAX) |
|---|---|
* 10 μl of PMS/reaction for 20 μl final volume
* Z: W, R, LR or F PMS, according to the qPCR instrument
* required number of reactions
Example: PMS1-W100 for Perfect Master Mix SYBR® Green for 100 reactions with the Light Cycler® 480 instrument
| Reference type : PMS1-Z2S | |
| PMS Perfect Master Mix SYBR® Green SignArrays® 96* | |
| Number of SignArrays® 96 | Price/unit (before TAX) |
|---|---|
* 10 μl of PMS/reaction for 20 μl final volume
* Z: W, R, LR or F PMS, according to the qPCR instrument
*2: according to the number of ordered Microbiota SignArray®
example: PMS1-W12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument
| Reference type : PMS2-Z2S | |
| PMS (Perfect Master Mix SYBR® Green) SignArrays® 384* | |
| Number of SignArrays® 384 | Price/unit (before TAX) |
|---|---|
* 5 μl of PMS/reaction for 10 μl final volumes
* Z: W, R, LR or F PMS, according to the qPCR instrument
*2: according to the number of ordered Microbiota SignArrays®
** Please request for an estimate at: [email protected]
example: PMS2-W12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument
AnyGenes® can help you select microbial targets, choose the appropriate assay format and build a microbiota qPCR workflow adapted to your sample type, host species, body site and research objective.
Whether your project involves gut microbiota, skin microbiota, oral microbiota, immune regulation, inflammation, cancer research or antibiotic resistance, our team can help define a targeted qPCR strategy.
Microbiota qPCR assays are used to detect and quantify selected microbial species or strains in targeted microbiome profiling workflows.
Microbiota qPCR assays focus on selected microbial targets, whereas 16S-based approaches rely on conserved bacterial regions and broader taxonomic profiling. Targeted qPCR can be useful when specific species or strains need to be analyzed.
Yes. AnyGenes® can prepare customized Microbiota SignArrays® based on selected microbial targets, host species, body site or research objective.
Microbiota qPCR workflows can be adapted to different sample types, including gut, skin, oral cavity or other microbiota-related biological samples, depending on DNA quality and extraction strategy.
Yes. Microbiota SignArrays® can be prepared in 96- or 384-well formats according to the number of targets, compatible qPCR instrument and project requirements.
Yes. AnyGenes® can help select microbial targets and design a qPCR panel according to your host species, body site, biological question and microbiota research objective.
Visit the AnyGenes® Help & FAQ section for detailed information about our products, services, ordering process and technical support.
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