Logout
If you want to log out click in LogOut
AnyGenes® provides a large collection of validated qPCR primers designed to support accurate and reproducible gene expression analysis in real-time PCR workflows.
Each primer pair is experimentally assessed for amplification efficiency, specificity and compatibility with AnyGenes® Perfect Master Mix SYBR® Green, helping researchers save time in assay design and qPCR validation.
AnyGenes® validated primer sets are designed for researchers who need reliable qPCR assays for targeted gene expression studies, pathway analysis and biomarker-focused projects.
Primer pairs are assessed under defined qPCR conditions to support reliable amplification.
Primer efficiency is experimentally checked, with expected efficiency typically between 90% and 110%.
Primer pairs are evaluated by melting curve analysis and agarose gel electrophoresis.
Intron-spanning designs are used whenever possible to help reduce genomic DNA amplification.
Primer sets are available for human, mouse, rat, pig and other species on demand.
Each validated primer set is provided as a 20x concentrate (20xOL) containing forward and reverse primers.
Validated qPCR primers help reduce the time required for primer design, assay testing and optimization. Each AnyGenes® primer pair is developed to support targeted amplification of selected gene transcripts in real-time PCR workflows.
Primer validation includes experimental assessment of amplification efficiency, specificity and performance with the AnyGenes® Perfect Master Mix SYBR® Green.
Primer efficiency is experimentally evaluated to support robust quantification across qPCR assays.
Primer pairs are tested for a single melting curve peak, supporting specific amplification.
Primer sets are checked for a single band at the expected size when applicable.
Primer efficiency is guaranteed by our rigourous experimental quality controls (efficiency between 90% and 110%) by using our Perfect Master Mix SYBR® Green (PMS) specifically developed to ensure you the best results in quantitative real-time PCR.
Based on strict intron spanning design (whenever possible, according to the genes of interest), all our qPCR primers are tested for a single peak on the melting curve and a single gel band at the expected weight. Each primer pair detects a specific product and guarantees the best specificity.
Our Perfect Master Mix SYBR® Green reagent (PMS) has been developed and validated with all our primer sets and signaling pathways for reliable and reproducible qPCR results (correlation between the same SignArrays® tested with same cDNAs).
AnyGenes® validated primer sets can support a broad range of targeted qPCR applications in molecular biology and biomarker research.
Quantify selected genes, transcripts or expression signatures using real-time PCR.
Use validated primer sets within AnyGenes® SignArrays® pathway-focused qPCR panels.
Confirm selected biomarker candidates after RNA-seq, screening or exploratory analysis.
Support copy number variation studies using dedicated genomic DNA qPCR assays.
Support targeted analysis of genomic regions in chromatin immunoprecipitation workflows.
Measure selected transcript isoforms using carefully designed qPCR assays.
Search for your gene of interest by gene symbol, gene name or RefSeq ID to identify available validated qPCR primer sets and associated signaling pathway panels.
For optimal results, AnyGenes® recommends combining validated qPCR primers with Perfect Master Mix SYBR® Green, as both products have been co-developed and tested together in real-time PCR workflows.
AnyGenes® validated qPCR primers can be combined with compatible qPCR reagents and personalized SignArrays® to support targeted gene expression analysis and biomarker validation projects.
Use validated primer sets with Perfect Master Mix SYBR® Green for dye-based real-time PCR workflows.
For probe-based detection, multiplex qPCR or TaqMan®-style assays, AnyGenes® can support suitable qPCR workflows.
Create custom qPCR panels based on selected genes of interest and your research objectives.
Specific gene expression assays are available as validated primer sets in flexible reaction formats. Each primer set is supplied as a 20× oligonucleotide mix containing forward and reverse primers.
Reference type: HE-XXXX-50
XXXX corresponds to the target gene name according to approved gene nomenclature. HE refers to human expression assays. M and R formats are used for mouse and rat assays.
Perfect Master Mix SYBR® Green can be ordered together with validated primer sets to support standardized qPCR workflows.
Reference type: PMS1-Z 50, with Z corresponding to the selected reference dye configuration.
Specific gene expression assays (validated primer sets) prices
| Reference type : HE-XXXX–50 | |
| Validated Primer sets | |
| Number of Reactions | Price (before TAX) |
|---|---|
*XXXX = the gene name (according to the HGNC approved-gene nomenclature)
* required number of reactions
* HE for Human expression, HCN for Human gDNA CNV, or HP for Human gDNA promoter applications
and M for Mus musculus species instead of H or R for Rattus norvegicus species instead of H
PMS Reagents associated with gene expression assays
| Reference type : PMS1-Z50 | |
| Perfect Master Mix SYBRG® | |
| Number of Reactions | Price (before TAX) |
|---|---|
* 10 μl of PMS/reaction for 20 μl final volume
* required number of reactions
AnyGenes® combines qPCR assay design, experimental primer validation and pathway-focused gene expression expertise to support reliable molecular biology workflows.
Thousands of validated primer sets are available across genes, pathways and species.
Primer sets can be integrated into SignArrays® panels for signaling pathway and biomarker studies.
Experimentally validated primer pairs help researchers avoid time-consuming in-house assay validation.
Designed to work with AnyGenes® qPCR reagents, SignArrays® panels and data analysis tools.
Custom primer design and validation can be requested for specific genes, species or project needs.
AnyGenes® can help select primer sets, pathway panels and qPCR workflows adapted to your project.
AnyGenes® can help you identify available validated primer sets, select compatible qPCR reagents, design custom assays or build personalized SignArrays® panels for your research project.
Whether your project involves gene expression profiling, RNA-seq validation, signaling pathway analysis or biomarker research, our team can support your qPCR assay strategy.
Validated qPCR primers are experimentally tested primer pairs designed to amplify specific gene targets with assessed efficiency and specificity in real-time PCR workflows.
Validated primer sets can save time by reducing the need for in-house primer design, assay testing and validation. They can also improve workflow consistency when used under recommended qPCR conditions.
Primer pairs are experimentally assessed for amplification efficiency, melting curve specificity and expected amplicon size when applicable.
Validated qPCR primer sets are available for human, mouse, rat, pig and other species on demand.
Yes. AnyGenes® can provide custom primer design and validation for specific genes, species or research projects on request.
Yes. Probe-based qPCR assays can be provided on demand for projects requiring fluorescent probe detection.
Visit the AnyGenes® Help & FAQ section for detailed information about our products, services, ordering process and technical support.
Visit Help & FAQ