Unlocking the power of the nitric oxide pathway in cellular signaling

The nitric oxide pathway (NO) plays a critical role in regulating various physiological processes, including vasodilation, immune response, and neurotransmission. Nitric pathway acts as a signaling molecule impacting cellular communication, inflammation, and programmed cell death. Understanding this pathway is essential for research into cardiovascular diseases, cancer and neurological disorders.

At AnyGenes, we provide cutting-edge tools to support your research into the nitric oxide pathway. Our SignArray^® qPCR arrays are designed to offer comprehensive, high-throughput solutions for analyzing gene expression related to NO signaling. These arrays are customizable for multiple species, including human, mouse, rat and pig.

Discover our advanced qPCR arrays for Nitric Oxide Signaling pathway research.

The NOS pathway and potential effects of NO on cardiovascular,renal and metabolic functions.

Key biomarkers in the nitric oxide pathway

The biomarkers of the NO pathway are crucial for understanding diseases mechanisms. Here are the key biomarkers of this pathway:

Nitric oxide synthases (NOS): the enzymes responsible for NO production, such as NOS1 (nNOS) for neuronal signaling, NOS2 (iNOS) for immune responses, and NOS3 (eNOS) for endothelial and vascular function.
S-Nitrosylation: a modofication of proteins influenced by NO, regulating cell signaling and oxidative stress.
Cyclic GMP (cGMP): a messenger molecule activated by NO, essential for vascular relaxation and linked to cardiovascular health.
VEGF (Vascular Endothelial Growth Factor): NO regulates VEGF, acritical factor for angiogenesis and tissue repair.

Mechanisms driving the NO pathway

The NO pathway drives several key biological mechanisms that impact diseases and cellular health. These include:

NO-cGMP signaling: modulates smooth muscle relaxation and blood vessel dilatation, important for cardiovascular function.
Reactive Nitrogen Species (RNS): NO metabolites can cause oxidative damage, contributing to conditions like cancer and neurodegenerative diseases.
Heme Oxygenase-1 (HO-1): an enzyme induced by NO during oxidative stresss, offering cytoprotection and playing a role in inflammation regulation.

Nitric oxide pathway in immune response & inflammation

NO produced by immune cells like macrophages and neutrophils during infections or tissue damage, where it acts as a defense mechanism. Key aspects include:

Immune Modulation: NO helps to regulate the activity of immune cells, influencing processes like pathogen destruction and inflammation resolution.
Chronic inflammation: dysregulated NO production can contribute to rheumatoid arthritis, asthma and inflammatory bowel disease (IBD).
Targeting NO for immunotherapies: modulating NO levels is being explored as a potential strategy to balance immune responses in conditions of excessive or insufficient inflammation.

NO pathway in therapeutic applications

The NO pathway is key to various therapeutic areas:

Cardiovascular health: NO helps regulate blood pressure and vasodilation, making it a target for treatments of hypertension and heart diseases.
Cancer therapy: NO can both promote and inhibit tumor growth, with potential therapies using NOS inhibitors or donors to control cancer progression.
Neuroprotective: targeting NO pathways can reduce oxidative stress and inflammation, offering protection in neurodegenerative conditions.
Wound healing: NO aids angiogenesis and tissue repair, speeding up recovery from injuries through NO-based therapies.

Analyze your pathway data with AnyGenes® software

Scientific data is only as powerful as the analysis behind it.

AnyGenes® provides a dedicated data analysis tool specifically developed for SignArrays® pathway panels.

What does it allow you to do?

Automated ΔCq calculation
Normalization with selected housekeeping genes
Comparison of up to 10 experimental conditions
Generation of descriptive statistics
Publication-ready graphs
Exportable tables for manuscripts and presentations

Developed on Excel (compatible with 2007+), the software is user-friendly and requires no advanced bioinformatics skills.

Customize your own signaling pathways (SignArrays®) with the factors of your choice!
Simply download and complete our Personalized SignArrays® information file and send it at [email protected] to initiate your project.

Nitric oxyde signaling pathway biomarker list

You can check the biomarker list included in this pathway, see below:

Species : Homo sapiens (human)

Human Signaling Pathway

Signaling Pathways	Product ref	Informations
Nitric Oxide Signaling Pathway	NO1H1	Download biomarker list

Species : Mus musculus (mouse)

Mouse Signaling Pathway

Signaling Pathways	Product ref	Informations
Nitric Oxide Signaling Pathway	NO1M1	Download biomarker list

Species : Rattus norvegicus (rat)

Rat Signaling Pathway

Signaling Pathways	Product ref	Informations
Nitric Oxide Signaling Pathway	*******	Biomarker list in progress.

Species : Sus Scrofa (Pig)

Pig Signaling Pathway

Signaling Pathways	Product ref	Informations
Nitric Oxide Signaling Pathway	*******	Biomarker list in progress.

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Bibliography

1. Kopp-Scheinpflug C et al. Nitric Oxide Signaling in the Auditory Pathway. Front Neural Circuits. (2021);15:759342.

2. Carlström M. Nitric oxide signalling in kidney regulation and cardiometabolic health. Nat Rev Nephrol. (2021);17(9):575-590.

3. López-Sánchez LM et al. Nitric oxide and tumor metabolic reprogramming. Biochem Pharmacol. (2020);176:113769.

1. Pourbagher-Shahri AM et al. An Overview of NO Signaling Pathways in Aging. Molecules. (2021);26(15):4533.

2. Liu X et al. Protein Phosphorylation in Cancer: Role of Nitric Oxide Signaling Pathway. Biomolecules. (2021);11(7):1009.

3. Reina-Torres E et al. The vital role for nitric oxide in intraocular pressure homeostasis. Prog Retin Eye Res. (2021);83:100922.

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