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Mycoplasma contamination is one of the most common hidden threats in cell culture, making the use of a reliable mycoplasma test kit essential for early detection and routine laboratory quality control.
AnyGenes® provides MycoDiag®, a qPCR and PCR mycoplasma test kit designed for fast, sensitive and reliable detection of mycoplasma contamination directly from cell culture supernatants.
Mycoplasma are small bacteria without a cell wall that can contaminate cell cultures without obvious visible signs.
In contaminated cultures, mycoplasma can affect several biological and experimental parameters, including:
As a result, undetected contamination can lead to false or misleading experimental conclusions, especially in studies focused on gene expression, signaling pathways, inflammatory responses, RNA-seq validation, and biomarker discovery.
Routine testing with a sensitive mycoplasma detection kit is therefore essential to help maintain reliable and reproducible cell culture experiments.
PCR-based methods are widely used to detect mycoplasma contamination in cell culture.
The AnyGenes® MycoDiag mycoplasma test kit enables direct detection of Mycoplasma DNA from cell culture supernatants using Real-Time qPCR or EndPoint PCR.
MycoDiag is designed for laboratories that need a fast, sensitive, and easy-to-use solution for routine mycoplasma screening.
Key features include:
qPCR is a fast, sensitive, and reproducible method for detecting mycoplasma contamination in cell culture samples.
Compared with slower or less direct approaches, a qPCR mycoplasma test kit offers several advantages for routine laboratory screening:
This makes qPCR-based solutions such as the MycoDiag mycoplasma test kit particularly useful before critical experiments, including qPCR analysis, RNA-seq, transfection assays, cell-based assays, and biomarker studies.
A quick overview of the AnyGenes® MycoDiag® mycoplasma test kit for fast and reliable detection of mycoplasma contamination in cell culture supernatants.
AnyGenes® mycoplasma test kit for cell culture contamination screening.
Compatible with real-time qPCR and end-point PCR workflows.
Direct detection of mycoplasma DNA from culture supernatants.
Fast turnaround for routine mycoplasma screening and quality control.
Detects 25 common mycoplasma species found in contaminated cell cultures.
Helps detect PCR inhibitors and reduce the risk of false-negative results.
Suitable for research, biotechnology and cell culture quality control workflows.
Kit format should be selected according to the instrument compatibility file.
The MycoDiag® mycoplasma test kit provides reliable detection of mycoplasma DNA in cell culture supernatants using optimized primers and validated protocols.
It is designed to support laboratories that need a rapid and reproducible method to monitor cell culture contamination and protect experimental reliability.
The assay detects 25 common mycoplasma species associated with contaminated cell cultures, including:
Each MycoDiag® kit includes a complete control system to support reliable interpretation of results.
Used to validate amplification and confirm assay performance.
Used to monitor potential contamination during the PCR/qPCR workflow.
Used to detect PCR inhibitors and help avoid false-negative results.
For end-point PCR applications, agarose gel electrophoresis allows visual confirmation of amplification.
In case of mycoplasma contamination, bands between 121 and 230 bp can be observed after PCR amplification with the MycoDiag® mycoplasma test kit.
Example of qPCR amplification curves obtained with MycoDiag® controls and contaminated samples.
Example of end-point PCR visual confirmation by agarose gel electrophoresis.
Regular mycoplasma testing is essential to maintain reliable cell culture experiments.
It is recommended to test cell cultures:
Using a routine mycoplasma test kit helps laboratories detect contamination early and reduce the risk of losing valuable samples, time, and experimental data.
Mycoplasma contamination can significantly affect biological results.
For laboratories working on gene expression, qPCR, RNA-seq, signaling pathways, inflammation, metabolism, or biomarker discovery, contamination can introduce bias and lead to incorrect biological interpretation.
Using a reliable mycoplasma test kit such as MycoDiag helps researchers:
This makes mycoplasma screening a critical step in molecular biology and cell culture workflows.
The AnyGenes® MycoDiag® mycoplasma test kit provides a practical and reliable solution for routine contamination screening in research, biotechnology and quality control laboratories.
Designed for qPCR and end-point PCR workflows, MycoDiag® helps laboratories detect mycoplasma contamination quickly and protect the reliability of cell culture experiments.
Visual summary of the main MycoDiag® assay advantages.
Results available in less than 2 hours for rapid routine screening.
Designed to detect mycoplasma DNA reliably in cell culture supernatants.
Covers major mycoplasma species commonly found in contaminated cell cultures.
Positive, negative and internal controls help reduce the risk of false-negative results.
Compatible with real-time qPCR and end-point PCR workflows.
Suitable for research laboratories, biotechnology teams and quality control workflows.
The MycoDiag mycoplasma test kit is available in several formats according to the number of reactions required.
| Catalog Reference | Number of Reactions | Price (before TAX) |
|---|---|---|
Need a fast and reliable mycoplasma test kit for your laboratory? Contact the AnyGenes® team to request pricing, technical information, distributor details or protocol support.
Bicêtre Hospital, AP-HP“Our lab has been using the MycoDiag reagent for over a year to monitor Mycoplasma contamination. We appreciate the consistent quality and excellent support from AnyGenes.”Read the full testimonial on our home page
A mycoplasma test kit is a molecular biology tool used to detect mycoplasma contamination in cell cultures. The AnyGenes® MycoDiag® mycoplasma test kit uses qPCR or PCR technology to detect mycoplasma DNA directly from cell culture supernatants.
Mycoplasma contamination can be detected using PCR-based methods. qPCR-based methods such as MycoDiag® allow fast and sensitive detection of mycoplasma DNA directly from cell culture supernatants.
Yes. Mycoplasma contamination can alter gene expression and affect pathways related to inflammation, stress response, metabolism and cell growth. This is why routine mycoplasma testing is important before qPCR, RNA-seq, transfection assays and biomarker studies.
The MycoDiag® mycoplasma test kit provides results in less than 2 hours, supporting rapid routine screening of cell culture supernatants.
Cell cultures are commonly tested every 1 to 2 weeks in routine culture, and before critical experiments such as qPCR, RNA-seq, transfection assays, biomarker studies or cell-based assays.
The MycoDiag® kit detects 25 common mycoplasma species found in contaminated cell cultures, including M. hyorhinis, M. orale, M. fermentans, M. pneumoniae, M. arginini, M. hominis, Acholeplasma laidlawii, Spiroplasma and others.
Yes. The MycoDiag® assay is compatible with standard qPCR and PCR systems. The appropriate kit format should be selected according to the qPCR instrument compatibility file.
Yes. MycoDiag® is designed for routine mycoplasma screening in research laboratories, biotechnology laboratories and quality control workflows.