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AnyGenes® Perfect Master Mix SYBR Green is a ready-to-use real-time PCR reagent designed for SYBR® Green-based qPCR workflows requiring sensitivity, efficiency and reproducible amplification.
Developed for molecular biology laboratories working on gene expression analysis, biomarker validation, signaling pathway studies and microbiota research, this 2X qPCR formulation helps simplify reaction setup while supporting consistent real-time PCR performance.
Perfect Master Mix SYBR Green is designed for researchers who need a convenient SYBR® Green qPCR reagent for targeted real-time PCR applications.
Designed to simplify qPCR reaction setup and reduce preparation time.
Compatible with dye-based real-time PCR workflows using double-stranded DNA fluorescence detection.
Formulated with Hot Start polymerase to support specific and efficient amplification.
Available with ROX, Low ROX, fluorescein or dye-free formats according to qPCR instrument requirements.
Suitable for use with AnyGenes® validated primer sets, qPCR arrays and targeted assays.
Supports gene expression analysis, biomarker validation, microbiota studies, CNV and more.
Perfect Master Mix SYBR Green combines SYBR® Green detection chemistry, optimized buffer components and a Hot Start Taq polymerase to support efficient real-time PCR amplification.
This formulation is designed to help reduce non-specific signal and primer-dimer formation, while supporting clean amplification curves and consistent fluorescence detection.
The reagent can be used in routine qPCR experiments as well as targeted workflows involving gene expression analysis, validated primer sets, SignArrays® panels and selected biomarker candidates.
Some quantitative PCR projects involve challenging samples or complex target regions, including FFPE-derived nucleic acids, laser capture microdissection samples, low-input biological material or GC-rich target regions.
Perfect Master Mix SYBR Green is designed to support reliable amplification in SYBR® Green-based qPCR workflows. For these demanding applications, nucleic acid quality, primer design, assay specificity, cycling conditions and melt curve analysis remain essential for obtaining reproducible results.
Can support targeted qPCR workflows using fragmented or limited nucleic acids, when sample quality is assessed and primer design is adapted to the project.
Can be used in workflows involving limited biological material, especially when sample preparation or pre-amplification is adapted to the experimental context.
Can support amplification of selected GC-rich targets, with particular attention to primer design, PCR conditions and specificity assessment.
This SYBR® Green-based formulation is developed to support sensitive and reproducible amplification across a wide range of real-time PCR applications.
Supports efficient amplification in validated qPCR assays and targeted gene expression workflows.
Designed to support reliable detection of amplified DNA in SYBR® Green-based assays.
Optimized formulation helps limit primer-dimer formation and non-specific signal when assays are properly designed.
Can be used with AnyGenes® validated primer sets and custom qPCR workflows.
Available in configurations adapted to common real-time PCR instrument requirements.
Supports consistent amplification performance for molecular biology and biomarker research projects.
Perfect Master Mix SYBR Green can support multiple molecular biology applications where targeted qPCR analysis is required.
Measure selected genes, transcripts or expression signatures using real-time PCR.
Compatible with AnyGenes® SignArrays® technology for pathway-oriented qPCR studies.
Confirm selected candidate biomarkers using targeted qPCR workflows.
Support targeted detection of DNA regions in chromatin immunoprecipitation workflows.
Support copy number variation studies using quantitative real-time PCR.
Measure selected transcript isoforms using carefully designed primer-based assays.
Support quantitative analysis of microbial communities in targeted qPCR workflows.
Use SYBR® Green qPCR workflows for selected pathogen or microbial target detection.
Support quantitative monitoring of selected viral targets in research workflows.
Perfect Master Mix SYBR Green is available in different reference dye configurations to match common qPCR instrument requirements.
For instruments requiring passive ROX reference dye.
For qPCR platforms requiring lower passive reference dye concentration.
For systems requiring fluorescein or workflows without passive reference dye.
Perfect Master Mix SYBR Green is available in multiple reaction sizes to support routine, medium-throughput and larger qPCR projects.
Reference type: PMS1-Z 50
Z corresponds to the selected reference dye configuration: W, R, LR or F, according to qPCR instrument compatibility.
Recommended use: 10 µl of PMS per reaction for a 20 µl final reaction volume.
Select the appropriate reaction number according to project size and experimental design.
| Reference type : PMS1-Z50 | |
| Perfect Master Mix SYBRG® | |
| Number of Reactions | Price/unit (before TAX) |
|---|---|
* 10 μl of PMS/reaction for 20 μl final volume
* Z : W, R, LR or F Perfect Master Mix SYBR® Green, according to the qPCR instrument
AnyGenes® develops qPCR reagents, validated primer sets and qPCR arrays for researchers working on gene expression analysis, signaling pathways and biomarker-focused projects.
Developed by a team experienced in qPCR workflows, gene expression analysis and molecular assay design.
Compatible with AnyGenes® validated primers, SignArrays® and targeted biomarker workflows.
Available in reference dye configurations adapted to common real-time PCR platforms.
Suitable for workflows involving gene expression, microbiota, CNV, ChIP-qPCR or selected low-input projects.
Support for choosing the appropriate format, compatible workflows and qPCR experimental strategy.
AnyGenes® technologies have been referenced in peer-reviewed publications across molecular biology research fields.
AnyGenes® reagents and related technologies have been referenced in peer-reviewed scientific publications, including journals such as Nature, Circulation and PLOS ONE.
These publications illustrate the use of AnyGenes® technologies in molecular biology, gene expression analysis and biomarker-focused research workflows.
AnyGenes® can help you select the appropriate reference dye configuration, validate your qPCR workflow and choose complementary solutions such as validated primers, SignArrays® or qPCR arrays.
Whether your project involves gene expression analysis, microbiota characterization, biomarker validation or targeted molecular detection, our team can help you identify the most suitable format.
Perfect Master Mix SYBR Green is a ready-to-use reagent for SYBR® Green-based real-time PCR. It can support gene expression analysis, biomarker validation, microbiota studies, CNV analysis, ChIP-qPCR and other targeted molecular biology workflows.
It is available with different reference dye configurations, including ROX, Low ROX, fluorescein or dye-free options. The correct format should be selected according to your instrument compatibility file.
The reagent is designed to simplify qPCR setup. However, primer design, assay specificity, sample quality and instrument settings remain important for reliable amplification results.
Yes. Perfect Master Mix SYBR Green can be used with AnyGenes® validated primer sets, targeted qPCR assays and SignArrays® workflows.
Yes. SYBR® Green qPCR workflows can support targeted microbiota characterization when assays are properly designed and validated.
You can contact the AnyGenes® team to request pricing, product information, compatibility guidance or support for your qPCR project.
Visit the AnyGenes® Help & FAQ section for detailed information about our products, services, ordering process and technical support.
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