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The AnyGenes® SpeAmp® qPCR preamplification kit improves sensitivity and reproducibility for low RNA samples, low-expression genes, and challenging transcriptomic workflows.
From as little as 5 ng of RNA, SpeAmp® enables reliable cDNA preamplification before qPCR and qPCR array analysis while preserving biologically relevant gene expression profiles.
Key benefits
Compatible with SignArrays® pathway panels and RNA-seq validation workflows.
Many transcriptomic experiments are limited by insufficient RNA quantity or weak transcript abundance.
Low-input RNA samples frequently generate:
These challenges are especially common in:
qPCR preamplification improves sensitivity by amplifying cDNA targets before real-time PCR analysis while maintaining reliable expression patterns across genes and pathways.
This approach enables more reproducible qPCR and qPCR array workflows from difficult biological samples.
SpeAmp® is a validated cDNA preamplification system developed by AnyGenes® for pathway-focused qPCR analysis and low-input RNA workflows.
The system supports:
Unlike non-optimized amplification workflows, SpeAmp® has been experimentally validated to preserve strong correlation between preamplified and non-preamplified samples.
This ensures reliable downstream biological interpretation after the preamplification step.
SpeAmp® maintains strong correlation and reproducibility across independent preamplification experiments, including low-expression genes and low-input RNA samples.
High correlation after preamplification
Validated experiments demonstrated strong correlation between preamplified and non-preamplified samples, supporting reliable biological interpretation after cDNA preamplification.
Key validation results
Validation highlight
R² > 0.99
Reproducible amplification across experiments
Independent experiments confirmed highly reproducible amplification performance for pathway-focused qPCR analysis and low-input transcriptomic workflows.
Validation highlight
Validated across 20+ independent experiments.
SpeAmp® is fully compatible with:
Researchers can customize their preamplification workflow using Primnx primer pools targeting:
This flexibility enables scalable and reproducible qPCR workflows adapted to advanced molecular biology projects.
SpeAmp® is suitable for multiple molecular biology and transcriptomic applications:
The system helps researchers obtain robust and biologically relevant data from challenging experimental material.
Works from only 5 ng of RNA
Compatible with 368 genes or 4 signaling pathways
Specific, customizable, and cost-effective
Suitable for Frozen tissues, biopsies, FFPE samples, LCM samples, PBMC…
Highly reproducible results validated through strict quality control
Scalable for large transcriptomic workflows
| Catalog Reference | Number of Reactions | Price |
|---|---|---|
XXXX : name of the selected specific signaling pathway
example : AN1H for human angiogenesis 1
| Catalog Reference | Number of Reactions | Price |
|---|---|---|
A qPCR preamplification kit amplifies small quantities of cDNA before real-time PCR analysis to improve sensitivity and reproducibility for low RNA samples and weakly expressed genes.
Low-input RNA samples often produce weak or inconsistent amplification signals. Preamplification increases target abundance before qPCR analysis, improving detection sensitivity and experimental consistency.
Yes. SpeAmp® is fully compatible with AnyGenes® SignArrays® signaling pathway panels and validated for pathway-focused gene expression analysis.
SpeAmp® supports: FFPE samples, biopsies, PBMCs, frozen tissues, LCM samples, single-cell material ..
Yes. Primnx pooled primer systems allow customization of amplification targets according to genes, biomarkers, or signaling pathways of interest.
Need to improve qPCR sensitivity from limited RNA samples? The AnyGenes® SpeAmp® qPCR preamplification kit helps researchers obtain reliable and reproducible gene expression data from difficult biological material. Whether you are performing: pathway analysis, RNA-seq validation, LncRNA profiling, biomarker discovery, single-cell transcriptomics... our technical experts can help you build the most appropriate qPCR workflow for your project.