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qPCR preamplification kit to improve your real-time PCR results

Boost your qPCR sensitivity and accuracy with the AnyGenes® qPCR preamplification kit (SpeAmp® system).

From as little as 5 ng of RNA, achieve reliable and reproducible gene expression results, even from challenging low-RNA or single-cell samples, fully compatible with SignArrays® panels.

single cell analysis. You can now analyse until 4 signaling pathways from only 5ng of RNA using the cDNA Preamplification SpeAmpn kit.

You have a small amount of biological sample but many signaling pathways to analyze

The AnyGenes® qPCR preamplification kit (SpeAmp® system) is the ideal solution to enhance your qPCR performance, even from low RNA or single-cell samples.

Our specific cDNA preamplification reagents enable high-throughput and reliable gene expression profiling from as little as 5 ng of RNA.

Boost your qPCR accuracy today - Request a free consultation with our experts.

Why choose the SpeAmp® qPCR preamplification kit ?

  • Perform up to 368 gene analyses or 4 signaling pathways using AnyGenes® SignArrays® panels.
  • Maintain accuracy and reproducibility for low-expression genes.
  • Fully compatible with SignArrays® signaling pathway profiling systems.
  • Customizable with Primnx primer pools targeting genes of your interest.
  • Perfect for single-cell profiling, biopsies, or FFPE samples.

Optimize every step of your qPCR workflow – from preamplification to data analysis.

Boost your qPCR sensitivity. Request a free consultation with our technical experts

High-performance reagents for efficient cDNA preamplification

Each preamplification kit SpeAmp® includes:

  • Perfect Master Mix, thermostable Taq DNA Polymerase, optimized buffer, MgCl₂, and dNTPs for high-efficiency preamplification.
  • AmpPuren, enzymatic purification mix for clean PCR products after preamplification.
  • Primnx (optional), primer pool corresponding to your chosen SignArray® pathway or custom gene list. Please order separately.

NB: The Primnx pool must correspond to the catalog reference of your selected SignArray®.

Discover the full qPCR reagent range. View the product catalog

Reproducibility and reliability for qPCR research

Our  SpeAmp® kit is experimentally validated by strict AnyGenes® quality control protocols :

Access our complete preamplification SpeAmp® kit guide

Start your preamplification project today !

Ready to achieve more from less RNA?

The AnyGenes® SpeAmp® kit gives you the precision, reproducibility, and confidence needed for advanced molecular biology projects.

Boost your researchs today. Contact our experts for tailored support or request a free consultation

Validated process with qPCR preamplification kit ensuring accuracy and correlation

Correlation

SpeAmpn reagents are validated to ensure very high correlation between experiments performed with and without cDNA Preamplification.
qPCR arrays results obtained after SpeAmpn cDNA Preamplification step are more reliable and easily interpretable, especially for low expressed genes.
  • The SpeAmp® kit guarantees high correlation between experiments with and without preamplification.
  • Data obtained after the preamplification step are reliable, reproducible, and statistically consistent, even for low-expressed genes.
  • Consistent expression levels after preamplification.
  • Validated on human and mouse RNA from diverse tissues

Reproducibility

preamplification cDNA: SpeAmpn reagents have been optimised to give you reproducible qPCR results.

  • High reproducibility of the preamplification SpeAmp® kit confirmed over 20 independent experiments.

Advantages of the SpeAmp® qPCR preamplification kit

Works from only 5 ng of RNA
Compatible with 368 genes or 4 signaling pathways
Specific, customizable, and cost-effective
Suitable for all sample types (Frozen tissues, biopsies, FFPE samples, LCM samples, PBMC…)
Highly reliable and reproducible results validated by our strict quality control procedure
Very attractive prices

Discover how SpeAmp® simplifies your data analysis and enhances reproducibility.

Contact our team or request a quote

cDNA Pre-Amplification kit

Catalog
Reference		Number
of Reactions		Price
SpeAmp® – 10
10
***
SpeAmp® – 25
25
***
SpeAmp® – 50
50
***

XXXX : name of the selected specific signaling pathway

example : AN1H for human angiogenesis 1

Pooled primers for cDNA Pre-Amplification kits

Catalog
Reference		Number
of Reactions		Price
Primnx-10 XXXX
10
***
Primnx-25 XXXX
25
***
Primnx-50 XXXX
50
***
Need pricing information or distributor details?
For quotations, product information, or project discussions, please contact our team at [email protected].

Frequently asked questions about qPCR preamplification kit SpeAmp®

What is a qPCR preamplification kit?

A qPCR preamplification kit is used to amplify small quantities of cDNA before real-time PCR analysis.
It increases sensitivity and reproducibility, especially for samples with low RNA content, such as single-cell, biopsy, or FFPE samples.

What are the benefits of the AnyGenes® SpeAmp® qPCR preamplification kit?

The AnyGenes® SpeAmp® kit offers specific, reproducible, and customizable cDNA preamplification.
It enables the analysis of up to 368 genes or 4 signaling pathways from only 5 ng of RNA, ensuring accurate gene expression data for low-yield samples.

Is the SpeAmp® kit compatible with SignArrays® panels?

Yes. The SpeAmp® system is fully compatible with AnyGenes® SignArrays® signaling pathway panels and has been validated to maintain high correlation between experiments with and without preamplification.

Which sample types are suitable for the SpeAmp® qPCR preamplification kit?

SpeAmp® reagents can be used with various sample types, including:

  • Frozen tissues
  • FFPE samples
  • PBMCs
  • Biopsies
  • LCM samples

These reagents ensure consistent and reliable amplification across all sample types.

Can I customize my preamplification with specific genes of interest?

Yes. By using Primnx primer pools, you can design your preamplification to target specific genes or signaling pathways of interest, allowing customized profiling that fits your research needs.

Looking for more answers? Visit our Help & FAQ section to find detailed informations about our products, services, and technical support.