Hi, Continue visiting your profile
user

Login

Email

Password

Create account
Forget password
AnyGenes
  • email login

Validated qPCR primers for reliable gene expression analysis

AnyGenes® provides an extensive collection of validated qPCR primers designed for accurate and reproducible gene expression quantification.

Each primer pair has been experimentally optimized and validated with our Perfect Master Mix SYBR® Green (PMS) to ensure unmatched performance in quantitative real-time PCR (qPCR) assays.

Boost your qPCR performance today. Request a free consultation with our experts.

Validated qPCR primers for precise results-AnyGenes

Large database of experimentally validated qPCR primers

Our database includes thousands of validated primer sets covering a wide range of genes and species (human, mouse, rat, and more).

Each validated primer set is provided 20× concentrated (20xOL), ready to use, and includes both forward and reverse primers optimized for targeted gene expression analysis.

  • Designed under strict experimental parameters
  • Compatible with most qPCR instruments
  • Supplied in flexible assay formats
  • Available at competitive prices

Why choose validated qPCR primers from AnyGenes®

Our validated qPCR primers undergo rigorous quality control and performance validation, guaranteeing consistent and reproducible results across experiments.

Efficiency and specificity

  • Primer efficiency between 90% and 110%, verified experimentally.
  • Intron-spanning design (whenever possible) ensures genomic DNA exclusion.
  • Each primer pair produces a single peak in melting curves and a single band on agarose gel electrophoresis.

Reproducibility and reliability

Each of our validated qPCR primers set is guaranteed by strict experimental quality controls (efficiency between 90% and 110%).

Primer efficiency is guaranteed by our rigourous experimental quality controls (efficiency between 90% and 110%) by using our Perfect Master Mix SYBR® Green (PMS) specifically developed to ensure you the best results in quantitative real-time PCR.

Based on strict intron spanning design (whenever possible, according to the genes of interest), all our qPCR primers are tested for a single peak on the melting curve and a single gel band at the expected weight. Each primer pair detects a specific product and guarantees the best specificity.

All of our validated qPCR primers sets are designed according to stringent intron spanning parameters and experimentally validated by strict quality controls.

AnyGenes monitors the reproducibility of their SignArrays with strict quality controls (example with 2 Human Apoptosis SignArrays performed from the same sample).

Our Perfect Master Mix SYBR® Green reagent (PMS) has been developed and validated with all our primer sets and signaling pathways for reliable and reproducible qPCR results (correlation between the same SignArrays® tested with same cDNAs).

NB: Probe assays are also available on demand.

Applications and compatible species

Our validated qPCR primers are suitable for a wide range of quantitative gene expression applications, including:
  • Gene expression profiling
  • Pathway analysis (SignArrays®)
  • CNV (copy number variation) detection
  • ChIP-qPCR assays
  • Microbiota and pathogen quantification
  • Splice variant analysis
Available species: Human • Mouse • Rat • Pig • Other species on demand

Search your favorite gene assays and associated signaling pathways

Search your gene of interest using the query tool below, by gene symbol, name, or RefSeq ID.

For optimal results, we recommend using our Perfect Master Mix SYBR® Green (PMS) reagents with validated qPCR primers.

Both products have been co-developed and validated together for maximum performance in real-time PCR.


Species :


Search by :



Customize your own signaling pathways (SignArrays®) with the factors of your choice!
Simply download and complete our Personalized SignArrays® information file and send it at [email protected] to get started on your project.

Specific gene expression assays

Specific gene expression assays (validated primer sets) prices

Reference type : HE-XXXX50
Validated Primer sets
Number of
Reactions
Price
(before TAX)
50
***
100
***
200
***
500
***

*XXXX = the gene name (according to the HGNC approved-gene nomenclature)

* required number of reactions

* HE for Human expression, HCN for Human gDNA CNV, or HP for Human gDNA promoter applications

and M for Mus musculus species instead of H or R for Rattus norvegicus species instead of H

PMS Reagents associated with gene expression assays

Reference type : PMS1-Z50
Perfect Master Mix SYBRG®
Number of
Reactions
Price
(before TAX)
50
***
100
***
200
***
500
***

* 10 μl of PMS/reaction for 20 μl final volume

* required number of reactions 

For detailed pricing and international orders, please contact us :

These prices are available only in FRANCE,

Frequently asked questions about validated qPCR primers

Validated qPCR primers are experimentally tested primer pairs designed to amplify specific gene targets with proven efficiency and specificity. They eliminate the need for in-house primer design and validation.

Validated primers from AnyGenes® save time and ensure reliable, reproducible results. Each primer set is optimized for efficiency, melting temperature, and compatibility with our master mixes.

Yes. AnyGenes® offers custom primer design and validation for any gene of interest across human, mouse, rat, and other species.

Yes. Probe-based qPCR assays can be provided on demand upon request.

Need more details ? Explore our Help & FAQ center for in-depth answers about AnyGenes® products, applications, and technical support.