Validated qPCR primers for reliable gene expression analysis
AnyGenes® provides an extensive collection of validated qPCR primers designed for accurate and reproducible gene expression quantification.
Each primer pair has been experimentally optimized and validated with our Perfect Master Mix SYBR® Green (PMS) to ensure unmatched performance in quantitative real-time PCR (qPCR) assays.
Large database of experimentally validated qPCR primers
Our database includes thousands of validated primer sets covering a wide range of genes and species (human, mouse, rat, and more).
Each validated primer set is provided 20× concentrated (20xOL), ready to use, and includes both forward and reverse primers optimized for targeted gene expression analysis.
Our validated qPCR primers undergo rigorous quality control and performance validation, guaranteeing consistent and reproducible results across experiments.
Efficiency and specificity
Primer efficiency between 90% and 110%, verified experimentally.
Intron-spanning design (whenever possible) ensures genomic DNA exclusion.
Each primer pair produces a single peak in melting curves and a single band on agarose gel electrophoresis.
Reproducibility and reliability
Each primer set is validated using the Perfect Master Mix SYBR® Green, ensuring standardization and multiplexing compatibility.
Tested across SignArrays® pathway panels, demonstrating strong correlation and reproducibility.
Primer efficiency is guaranteed by our rigourous experimental quality controls (efficiency between 90% and 110%) by using our Perfect Master Mix SYBR® Green (PMS) specifically developed to ensure you the best results in quantitative real-time PCR.
Based on strict intron spanning design (whenever possible, according to the genes of interest), all our qPCR primers are tested for a single peak on the melting curve and a single gel band at the expected weight. Each primer pair detects a specific product and guarantees the best specificity.
Our Perfect Master Mix SYBR® Green reagent (PMS) has been developed and validated with all our primer sets and signaling pathways for reliable and reproducible qPCR results (correlation between the same SignArrays® tested with same cDNAs).
NB: Probe assays are also available on demand.
Applications and compatible species
Our validated qPCR primers are suitable for a wide range of quantitative gene expression applications, including:
PMS Reagents associated with gene expression assays
Reference type : PMS1-Z50
Perfect Master Mix SYBRG®
Number of Reactions
Price (before TAX)
50
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100
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200
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500
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* 10 μl of PMS/reaction for 20 μl final volume
* required number of reactions
Need pricing information or distributor details?
For quotations, product information, or project discussions, please contact our team at
[email protected].
Frequently asked questions about validated qPCR primers
What are validated qPCR primers?
Validated qPCR primers are experimentally tested primer pairs designed to amplify specific gene targets with proven efficiency and specificity. They eliminate the need for in-house primer design and validation.
Why use validated primers instead of designing my own?
Validated primers from AnyGenes® save time and ensure reliable, reproducible results. Each primer set is optimized for efficiency, melting temperature, and compatibility with our master mixes.
Can I request primers for a specific gene or species?
Yes. AnyGenes® offers custom primer design and validation for any gene of interest across human, mouse, rat, and other species.
Are probe-based assays also available?
Yes. Probe-based qPCR assays can be provided on demand upon request.
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