AnyGenes® RNA-seq qPCR validation

Q: How many genes should be validated after RNA-seq?

Most studies validate 5 to 20 genes depending on biological relevance and differential expression results.

Q: Why is qPCR used to validate RNA-seq results?

qPCR provides highly sensitive, independent confirmation of RNA-seq gene expression changes, increasing the reliability of transcriptomic findings.

Q: Do I need to provide extra RNAfor qPCR validation?

No. AnyGenes® performs qPCR validation directly from the initial high-quality RNAextraction.

Q: Which pathways can be validated with AnyGenes® qPCR arrays?

AnyGenes® qPCR arrays cover more than 350 biological pathways including immunity, oncology, inflammation, cell signaling, apoptosis, angiogenesis and more.

Q: Are the results publication-ready?

Yes. All deliverables include figures, statistical analyses and methodology sections suitable for publication.

High-confidence transcriptomic analysis powered by integrated RNA-seq and qPCR validation

AnyGenes^®provides an advanced end-to-end platform dedicated to RNA-seq qPCR validation, offering exceptional accuracy, reproducibility, and scientific robustness. Our fully in-house workflow takes your samples seamlessly from raw biological material to validated gene expression insights, without multiple suppliers, hidden variability, or additional RNA extraction steps.

Our approach combines Illumina NGS expertise with proprietary qPCR arrays, ensuring reliable confirmation of biomarkers, pathway signatures, and differential expression profiles, using :

mRNA-seq
LncRNA-seq
Small RNA-seq
CircRNA-seq
Exosome RNA-seq

A complete, all-inclusive RNA-seq validation workflow

1. Sample reception & expert handling

We process human, mouse, rat and cell-culture samples under strict traceability and QC. Each sample is logged, inspected, and stabilized to preserve RNAquality from the first minute.

2. Optimized RNA extraction for every sample type

High-yield, high-purity extraction protocols adapted to:

fresh or frozen tissues
blood and plasma
cell pellets
primary and immortalized cells

Our extraction procedures ensure reliable input for downstream RNA-seq and qPCR validation.

3. Advanced RNA quality control

Every RNA sample is evaluated using:

RIN/RQN integrity scoring
260/280 and 260/230 purity ratios
concentration assessment
inhibitory compound screening

Only high-quality RNA proceeds to library preparation.

4. NGS library construction & quality control

We generate high-complexity Illumina-compatible libraries using validated protocols. Our QC pipeline includes:

Bioanalyzer / TapeStation profiling
qPCR-based library quantification
contamination and adapter-dimer checks

5. High-performance illumina sequencing

Sequencing is performed on high-throughput Illumina platforms with customizable read depth and read length (SE/PE). Coverage is tailored to your scientific objectives (biomarkers, pathways, differential expression, signature modeling).

6. Comprehensive bioinformatics pipeline

Our standardized and publication-ready pipeline includes:

FastQC / MultiQC reporting
adapter trimming
alignment (STAR, HISAT2)
gene expression quantification
differential expression analysis
pathway and GO enrichment
biomarker identification and prioritization

Delivered with clear visualizations and interpretable insights.

7. Biomarker validation using AnyGenes® qPCR arrays

To maximize confidence in your transcriptomic results, AnyGenes^®performs orthogonal validation of RNA-seq results using targeted qPCR arrays:

confirmation of key up/down-regulated genes
high-precision quantification
pathway-specific validation (oncology, inflammation, immunity, cell signaling…)
targeted biomarker set the most significant and relevant to your project from your RNA-seq data results
no additional RNA extraction required
enhanced reproducibility for translational and clinical studies

Why choose AnyGenes® for RNA-seq qPCR validation?

Fully controlled in-house NGS + qPCR workflow
Optimized RNA extraction and QC
High-accuracy Illumina sequencing
Expert bioinformatics for biomarker research
Proprietary qPCR arrays for powerful validation
Trusted by academic, biotech, pharmaceutical, and clinical partners worldwide
Technologies cited in major journals (Nature, Circulation, PLoS One)

Applications of RNA-seq qPCR verification

Precision biomarker discovery

Validate biomarker signatures for oncology, immunology, inflammation, regenerative medicine, and metabolic diseases.

Pathway-focused transcriptomic profiling

Confirm pathway activation using AnyGenes® cell-signaling qPCR arrays.

Clinical translational research

Strengthen the reliability and reproducibility of RNA-seq findings for preclinical and clinical studies.

Frequently asked questions: RNA-seq qPCR validation and expression confirmation

How many genes should be validated after RNA-seq ?

Typically 5-84 genes or more depending on the scope, variability, and biological significance of the study.

Why is qPCR used to validate RNA-seq results?

qPCR provides accurate, sensitive, and independent confirmation of gene expression changes detected by RNA-seq.

Do I need to provide extra RNA for qPCR validation?

No. AnyGenes® uses the same high-quality RNA extracted initially, avoiding additional sample processing.

Which pathways can be validated with AnyGenes® qPCR arrays?

Over 350 signaling pathways including inflammation, immunity, oncology, apoptosis, angiogenesis, cell cycle, stemness, metabolic pathways, and more.

Are the results publication-ready?

Yes. All analyses include figures, statistics, and methods suitable for jouRNAl submission.

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