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AnyGenes® Direct Lysis reagents (DiLysis kits) provide a fast, extraction-free workflow for single-cell RT-qPCR and gene expression analysis.
From as few as 10 cells, you can obtain precise, reproducible, and sensitive results, ideal for transcriptomic profiling and cell signaling pathway studies
The Direct Lysis qPCR reagents developed by AnyGenes® enable researchers to perform RT-qPCR directly from cell lysates – no RNA extraction required.
This innovative formulation preserves RNA integrity, minimizes sample loss, and accelerates your workflow
For enhanced sensitivity and reproducibility, combine with the SpeAmp® cDNA preamplification kit.
Developed by the AnyGenes® scientific team, the DiLysis kits are widely used in biomarker discovery and single-cell signaling pathway analysis.
Multiple studies confirm the robustness and accuracy of direct lysis + RT-qPCR approaches for low-input gene expression profiling :
Combining DiLysis kits with the SpeAmp® cDNA preamplification system allows detection of multiple gene targets even from very low cell numbers.
This powerful workflow enhances the sensitivity and scope of your single-cell RT-qPCR studies.
Applications:
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With over 20 years of expertise in quantitative real-time PCR, AnyGenes® collaborates with leading academic and clinical research laboratories worldwide.
Our SignArrays®, SpeAmp®, and Perfect Master Mix® technologies are trusted by researchers for their precision, reproducibility, and consistent performance.
Explore peer-reviewed articles, collaborative research, and citations featuring AnyGenes® technologies in journals such as Nature, PLoS ONE, and Circulation.
Ready to simplify your single-cell RT-qPCR workflow? Contact our experts to get your customized Direct Lysis (DiLysis) solution today.
Direct lysis reagents are designed to lyse cells directly, eliminating RNA extraction steps before RT-qPCR. They enable fast and efficient gene expression analysis, especially from small cell numbers.
Yes, AnyGenes® Direct Lysis reagents (DiLysis kits) are optimized for single-cell applications, allowing precise transcriptomic profiling from 10 to 100 cells.
The optimized buffer formulation prevents RNA degradation during lysis, ensuring high-quality templates for downstream RT-qPCR.
Combining the Direct Lysis reagents with AnyGenes® SpeAmp® preamplification system enhances detection sensitivity and reproducibility.
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