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AnyGenes® provides a complete, optimized workflow for single cell analysis, combining DiLysis (Direct Lysis), SpeAmp® (Specific Preamplification), and SignArrays® qPCR arrays.
This integrated system enables researchers to explore gene expression and signaling pathways from as few as 10 cells with outstanding sensitivity and reproducibility.
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qPCR at the single-cell level delivers the precision, sensitivity, and flexibility needed to study gene expression in the individual cell.
To obtain robust and reproducible results, each step, from experimental design to data analysis, must be optimized: single-cell collection, direct lysis, reverse transcription, preamplification, qPCR, and interpretation.
To meet the challenges of limited biological material, AnyGenes® has developed DiLysis and SpeAmp® reagent ranges providing a complete solution for single cell profiling and signaling pathway analysis.
Our reagents are fully compatible with the SignArrays® system, enabling exploration of multiple signaling pathways from just a few cells or nanograms of RNA.
With practical packaging starting from 10 reactions, researchers can easily test or optimize their single cell qPCR experiments.
Already cited in major scientific journals such as Nature, Circulation, and PLoS One, AnyGenes® qPCR reagents ensure publication-grade reproducibility and reliability.
SpeAmp® reagents enable efficient and specific preamplification for rare or low-input RNA samples.
The direct lysis step rapidly produces high-quality lysates without RNA loss compared to traditional extraction.
The optimized DiLysis formulation ensures:
With DiLysis, you can perform signaling pathway analysis from only 10-100 cells, ideal for rare samples.
By combining DiLysis, SpeAmp®, and SignArrays®, AnyGenes® delivers a comprehensive individual cell gene expression workflow ensuring :
Single cell analysis by qPCR enables researchers to quantify gene expression in individual cells, providing insight into cellular heterogeneity and signaling pathways.
Preamplification increases the amount of cDNA from low RNA inputs, improving sensitivity and reproducibility for low-abundance genes.
Direct lysis avoids RNA loss and degradation, providing a faster and more efficient workflow for single cell or low-input qPCR experiments.
Yes. DiLysis, SpeAmp®, and SignArrays® reagents are compatible with most qPCR platforms (Applied Biosystems, Bio-Rad, Qiagen, etc.).
Need more details ? Explore our Help & FAQ center for in-depth answers about AnyGenes® products, applications, and technical support.