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AnyGenes® shares its expertise in quantitative real-time PCR (qPCR) by developing optimized, ready-to-use qPCR master mixes designed for SYBR® Green (Perfect Master Mix SYBR Green) or TaqMan® probe chemistries (Perfect Master Mix PROBE).
Compatible with most qPCR instruments on the market and available with or without reference dyes (such as ROX or fluorescein), the Perfect Master Mixes developed by AnyGenes® guarantee maximum sensitivity, efficiency, and reproducibility – even when working with small amounts of cDNA or gDNA, or when amplifying challenging GC-rich sequences
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Each master mix contains a high-efficiency Hot Start Taq polymerase and all necessary buffer components. These real-time PCR reagents have been extensively validated on thousands of primers and probe sets within AnyGenes®’ high-throughput molecular biology platform.
They provide an all-in-one, plug-and-play solution for fast and simple use in any molecular biology laboratory. No optimization steps are required – each formulation has been carefully fine-tuned to deliver high amplification efficiency, even for low-copy targets, while minimizing non-specific hybridization and primer-dimer formation.
Every batch undergoes stringent quality control before shipping, guaranteeing consistent and reliable quantitative PCR performance.
The 2X qPCR master mixes developed by AnyGenes® are suitable for a wide range of research and diagnostic applications, including:
These reagents can also be combined with DiLysis (direct lysis) and/or SpeAmp® (preamplification) reagents to enable single-cell or few-cell profiling, providing exceptional sensitivity for limited samples.
Already cited in major scientific journals such as Nature, Circulation, and PLoS One, AnyGenes® qPCR reagents ensure publication-grade reproducibility and reliability.
Designed for SYBR® Green chemistry, this mix delivers high amplification efficiency and excellent reproducibility – ideal for routine or high-throughput gene expression studies.
Optimized for probe-based detection (TaqMan® chemistry), this mix provides superior specificity and robust amplification for precise quantification of target sequences.
AnyGenes® formulations are validated with thousands of assays, ensuring exceptional reproducibility and minimizing optimization time for researchers.
Yes. AnyGenes® qPCR master mixes are compatible with most major real-time PCR instruments, with options available with or without reference dyes (ROX or fluorescein).
Absolutely. These master mixes can be used with DiLysis™ and SpeAmpn™ reagents to ensure ultra-sensitive detection in low-copy or single-cell profiling experiments.
Need more details ? Explore our Help & FAQ center for in-depth answers about AnyGenes® products, applications, and technical support.