Rapid and reliable mycoplasma test kit for contamination-free cell cultures
Mycoplasma test kit (MycoDiag) for a mycoplasma-free cell culture !
Ensure the reliability of your research with fast and accurate detection of Mycoplasma contamination using AnyGenes® MycoDiag, a qPCR-based assay trusted by laboratories worldwide.
Ensure research accuracy with the MycoDiag mycoplasma test kit
Contamination of cell cultures by Mycoplasma is one of the most common and damaging problems in life science laboratories.
These small, wall-less bacteria are invisible under light microscopy, yet they can profoundly alter cell physiology, leading to:
impaired growth,
failed transfections,
morphological and phenotypic changes,
and altered gene expression profiles.
Such contamination can compromise the reliability and reproducibility of your experimental results.
To help researchers maintain a mycoplasma-free culture, AnyGenes® has developed MycoDiag, an advanced mycoplasma test kit designed for fast, sensitive, and reliable detection of contamination by qPCR or PCR.
Fast and accurate qPCR mycoplasma test kit detection
The MycoDiag mycoplasma test kit enables direct detection of Mycoplasma DNA from cell culture supernatants in less than two hours.
Compatible with both Real-Time qPCR and EndPoint PCR platforms, it provides a powerful solution for routine mycoplasma screening in research, biotechnology, and quality control laboratories.
Based on highly specific primers and validated through stringent quality control, MycoDiag ensures:
Exceptional sensitivity and specificity,
Reproducible performance across qPCR systems,
Accurate detection even at low contamination levels.
Comprehensive species detection with the MycoDiag
The MycoDiag assay detects the 25 most common Mycoplasma species found in contaminated cell cultures [2], including:
M. agalactiae
M. genitalium
M. pulmonis
M. arginini
M. hominis
M. salivarium
M. arthritidis
M. hyorhinis
M. spermatophilum
M. bovis
M. hyosynoviae
M. synoviae
M. cloacale
M. opalescens
M. timone
M. falconis
M. orale
Acholeplasma laidlawii
M. faucium
M. pirum
Spiroplasma
M. fermentans
M. pneumoniae M129
Ureaplasma urealyticum
M. gallisepticum
This broad detection range ensures comprehensive protection for your cell culture contamination control programs.
Rapid workflow with built-in quality assurance
The MycoDiag detection kit delivers confirmed results in under 2 hours.
Each kit includes:
Positive Control (Mycoplasma DNA)
Negative Control
Internal Control (to verify the absence of PCR inhibitors and eliminate false negatives)
With its ease of use and reliability, MycoDiag has become the preferred qPCR mycoplasma detection assay in many research institutions and biotech laboratories worldwide.
Advantages of the MycoDiag mycoplasma test kit
Ultra-fast analysis: Detects contamination in less than 2 hours
High sensitivity and specificity: Detects 25 major Mycoplasma species
Full control system: Avoids false negatives via positive, negative, and internal controls
Universal compatibility Works with all qPCR and PCRinstruments
Easy-to-use format: Ideal for routine testing and quality control
Validated technology: Trusted by research institutes and biotech companies
Over the past years, several research laboratories have adopted the MycoDiag mycoplasma test kit to ensure the quality and integrity of their cell cultures.
Users highlight the high reliability of the reagent, the fast delivery, and the responsiveness of the AnyGenes® team to technical inquiries.
“Our lab has been using the MycoDiag reagent for over a year to monitor Mycoplasma contamination. We appreciate the consistent quality and excellent support from AnyGenes.”
Tantibhedhyangkul W et al. Anti-Mycoplasma Activity of Daptomycin and Its Use for Mycoplasma Elimination in Cell Cultures of Rickettsiae. Antibiotics (Basel). (2019); 8(3): 123.
Mycoplasmas and tissue culture cells. (1992). In: Maniloff, J., McElhaney, R.N., Finch, L.R., Baseman, J.B. (Eds.), Mycoplasmas, Molecular Biology and Pathogenesis, American Society for Microbiology, 445-54.
A Mycoplasma test kit is a molecular biology tool used to detect Mycoplasma contamination in cell cultures. The MycoDiag kit by AnyGenes® uses qPCR or PCR technology for fast and sensitive detection.
The MycoDiag detection kit identifies 25 of the most frequent Mycoplasma species found in contaminated cultures, including M. hyorhinis, M. orale, M. fermentans, and M. pneumoniae.