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Rapid and reliable mycoplasma test kit for contamination-free cell cultures

Mycoplasma test kit (MycoDiag) for a mycoplasma-free cell culture !

Ensure the reliability of your research with fast and accurate detection of Mycoplasma contamination using AnyGenes® MycoDiag, a qPCR-based assay trusted by laboratories worldwide.

Ready to protect your research?

Order MycoDiag now!
Mycoplasma test kit MycoDiag ensure a fast and reliable screening of your cell culture supernatants for Mycoplasma contamination by qPCR or PCR .

Ensure research accuracy with the MycoDiag mycoplasma test kit

Contamination of cell cultures by Mycoplasma is one of the most common and damaging problems in life science laboratories.

These small, wall-less bacteria are invisible under light microscopy, yet they can profoundly alter cell physiology, leading to:

  • impaired growth,
  • failed transfections,
  • morphological and phenotypic changes,
  • and altered gene expression profiles.

Such contamination can compromise the reliability and reproducibility of your experimental results.

To help researchers maintain a mycoplasma-free culture, AnyGenes® has developed MycoDiag, an advanced mycoplasma test kit designed for fast, sensitive, and reliable detection of contamination by qPCR or PCR.

Fast and accurate qPCR mycoplasma
test kit detection

The MycoDiag mycoplasma test kit enables direct detection of Mycoplasma DNA from cell culture supernatants in less than two hours.

Compatible with both Real-Time qPCR and EndPoint PCR platforms, it provides a powerful solution for routine mycoplasma screening in research, biotechnology, and quality control laboratories.

Based on highly specific primers and validated through stringent quality control, MycoDiag ensures:

  • Exceptional sensitivity and specificity,
  • Reproducible performance across qPCR systems,
  • Accurate detection even at low contamination levels.

Comprehensive species detection with the MycoDiag

The MycoDiag assay detects the 25 most common Mycoplasma species found in contaminated cell cultures [2], including:

  • M. agalactiae
  • M. genitalium
  • M. pulmonis
  • M. arginini
  • M. hominis
  • M. salivarium
  • M. arthritidis
  • M. hyorhinis
  • M. spermatophilum
  • M. bovis
  • M. hyosynoviae
  • M. synoviae
  • M. cloacale
  • M. opalescens
  • M. timone
  • M. falconis
  • M. orale
  • Acholeplasma laidlawii
  • M. faucium
  • M. pirum
  • Spiroplasma
  • M. fermentans
  • M. pneumoniae M129
  • Ureaplasma urealyticum
  • M. gallisepticum

This broad detection range ensures comprehensive protection for your cell culture contamination control programs.

Rapid workflow with built-in quality assurance

The MycoDiag detection kit delivers confirmed results in under 2 hours.

Each kit includes:

  • Positive Control (Mycoplasma DNA)
  • Negative Control
  • Internal Control (to verify the absence of PCR inhibitors and eliminate false negatives)

With its ease of use and reliability, MycoDiag has become the preferred qPCR mycoplasma detection assay in many research institutions and biotech laboratories worldwide.

With highly sensitive and specific primer sets, mycoplasma test kit MycoDiag ensure the detection of Mycoplasma DNA in your samples by qPCR / PCR.
Agarose gel electrophoresis, after PCR with mycoplasma test kit MycoDiag, allows to observe bands between 121 to 230 bp, in case of Mycoplasma contamination of cell culture supernatants.

Advantages of the MycoDiag mycoplasma test kit

Compatible with all the qPCR / PCR instruments, these highly specific and sensitive easy-to-use mycoplasma test kit MycoDiag allow to detect Mycoplasma contamination of your cell cultures in less than 2 hours.
  • Ultra-fast analysis: Detects contamination in less than 2 hours
  • High sensitivity and specificity: Detects 25 major Mycoplasma species
  • Full control system: Avoids false negatives via positive, negative, and internal controls
  • Universal compatibility Works with all qPCR and PCR instruments
  • Easy-to-use format: Ideal for routine testing and quality control
  • Validated technology: Trusted by research institutes and biotech companies
  • Very competitive price

MycoDiag kit prices

These prices are available only in FRANCE.
Outside France?

Catalog Reference Number of Reactions Price (before TAX)
MycoDiag-X20
20
***
MycoDiag-X50
50
***
MycoDiag-X100
100
***

X : W, R, LR, F, according to the qPCR instrument or P for EndPoint PCR application (Please see our compatibility file)

Trusted by research teams worldwide

★ ★ ★ ★ ★

Over the past years, several research laboratories have adopted the MycoDiag mycoplasma test kit to ensure the quality and integrity of their cell cultures.

Users highlight the high reliability of the reagent, the fast delivery, and the responsiveness of the AnyGenes® team to technical inquiries.

“Our lab has been using the MycoDiag reagent for over a year to monitor Mycoplasma contamination. We appreciate the consistent quality and excellent support from AnyGenes.”
  1. Tantibhedhyangkul W et alAnti-Mycoplasma Activity of Daptomycin and Its Use for Mycoplasma Elimination in Cell Cultures of Rickettsiae. Antibiotics (Basel). (2019); 8(3): 123.
  2. Mycoplasmas and tissue culture cells. (1992). In: Maniloff, J., McElhaney, R.N., Finch, L.R., Baseman, J.B. (Eds.), Mycoplasmas, Molecular Biology and Pathogenesis, American Society for Microbiology, 445-54.

Frequently asked questions

A Mycoplasma test kit is a molecular biology tool used to detect Mycoplasma contamination in cell cultures. The MycoDiag kit by AnyGenes® uses qPCR or PCR technology for fast and sensitive detection.

The MycoDiag qPCR mycoplasma detection workflow provides results in less than 2 hours, allowing researchers to quickly verify cell culture purity.

The MycoDiag detection kit identifies 25 of the most frequent Mycoplasma species found in contaminated cultures, including M. hyorhinis, M. orale, M. fermentans, and M. pneumoniae.

Yes. The MycoDiag assay is compatible with all standard qPCR and PCR instruments, making it ideal for routine laboratory screening.

You can order directly by contacting AnyGenes® at [email protected] or through our authorized distributors worldwide.

Need more details ? Explore our Help & FAQ center for in-depth answers about AnyGenes® products, applications, and technical support.


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