The Microbiota Assays developed by AnyGenes® have been optimised with it range of Perfect Master Mix SYBR® Green to offer you a robust real-time qPCR system providing highly specific, reliable and reproducible results, even with small amounts of biological material, whatever the source of your samples.
All our microbiota assays are validated at the experimental level on a large collection of microbial DNA on our high-throughput molecular platform, according to stringent and strong quality control criteria. After validation in qPCR, each PCR amplicon is sequenced to ensure of the strict specificity of our microbiota primer sets to a unique microbial strain, contrary to 16S qPCR strategy that may induce non-specific amplification, due to highly conserved regions.
Our assays contains several internal controls to assess the high performance of the Microbiota Assays or SignArrays®, the quality of your samples, and the absence of DNA contamination.
These ready-to-use microbiota reagents are compatible with all:
Several formats are proposed (microbiota assays, Personalized Microbiota SignArrays® 96 or 384 plates), to better answer to each of your project issues.
To offer you a complete solution, AnyGenes® has generated a strong database of microbial strains on the basis of high impact factor publications implying high-throughput microbiota analysis. You can search in our database your favorite microbial strains to compose your own Microbiota qPCR Assays and SignArrays®:
| Reference type : MB–XXXX–100 | |
| Microbiota individual assays | |
| Number of Reactions | Price (before TAX) |
|---|---|
*XXXX = microbial species name (according to the NCBI Taxonomy Browser approved nomenclature)
* required number of reactions
* MB for Microbiota
Example: ME-B.vulgatus.8482-100 for the Bacteroides vulgatus ATCC 8482 species for 100 reactions
| Reference type : PZxHMB1-Y2S | |
| Customizable | |
| Number of SignArrays® 96 | Price/unit (before TAX) |
|---|---|
* Y: R, A, B or F plate type, according to the qPCR instrument (cf Compatibility file)
*2: according to the number of ordered Microbiota SignArrays®
example: PZxHMB1-R12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument
NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.
| Reference type : PZxHMB2-Y2S | |
| Customizable | |
| Number of SignArrays® 384 | Price/unit (before TAX) |
|---|---|
* Y: R, A, B or F plate type, according to the qPCR instrument
*2: according to the number of ordered Microbiota SignArrays®
example: PZxHMB2-R12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument
** Please request for an estimate at: [email protected]
NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.
| Reference type : PMS1-Z100 | |
| Perfect Master Mix SYBR® Green reagent (PMS) | |
| Number of Reactions | Price (before TAX) |
|---|---|
* 10 μl of PMS/reaction for 20 μl final volume
* Z: W, R, LR or F PMS, according to the qPCR instrument
* required number of reactions
Example: PMS1-W100 for Perfect Master Mix SYBR® Green for 100 reactions with the Light Cycler® 480 instrument
| Reference type : PMS1-Z2S | |
| PMS Perfect Master Mix SYBR® Green SignArrays® 96* | |
| Number of SignArrays® 96 | Price/unit (before TAX) |
|---|---|
* 10 μl of PMS/reaction for 20 μl final volume
* Z: W, R, LR or F PMS, according to the qPCR instrument
*2: according to the number of ordered Microbiota SignArray®
example: PMS1-W12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument
| Reference type : PMS2-Z2S | |
| PMS (Perfect Master Mix SYBR® Green) SignArrays® 384* | |
| Number of SignArrays® 384 | Price/unit (before TAX) |
|---|---|
* 5 μl of PMS/reaction for 10 μl final volumes
* Z: W, R, LR or F PMS, according to the qPCR instrument
*2: according to the number of ordered Microbiota SignArrays®
** Please request for an estimate at: [email protected]
example: PMS2-W12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument
All our assays are validated at the experimental level to guarantee you the best results.
