Hi, Continue visiting your profile
user

Login

Email

Password

Create account
Forget password
AnyGenes
  • email login

VALIDATED MICROBIOTA ASSAYS FOR HIGHEST QPCR PERFORMANCE

The Microbiota Assays developed by AnyGenes® have been optimised with it range of Perfect Master Mix SYBR® Green to offer you a robust real-time qPCR system providing highly specific, reliable and reproducible results, even with small amounts of biological material, whatever the source of your samples.

All our microbiota assays are validated at the experimental level on a large collection of microbial DNA on our high-throughput molecular platform, according to stringent and strong quality control criteria. After validation in qPCR, each PCR amplicon is sequenced to ensure of the strict specificity of our microbiota primer sets to a unique microbial strain, contrary to 16S qPCR strategy that may induce non-specific amplification, due to highly conserved regions.

Our assays contains several internal controls to assess the high performance of the Microbiota Assays or SignArrays®, the quality of your samples, and the absence of DNA contamination.

A STRONG QUANTITATIVE REAL-TIME PCR FLEXIBILITY WITH OUR MICROBIOTA ASSAYS

These ready-to-use microbiota reagents are compatible with:
– all sample types: gut, cutaneous, vaginal flora…
– all host species: Homo sapiens, laboratory models like Mus musculus or Rattus norvegicus…
– all qPCR instruments
– all microbiota application fields: involvement in cancer, immune system regulation, inflammation, homeostasis and antibiotic resistance.

Several formats are proposed (microbiota assays, Personalized Microbiota SignArrays® 96 or 384 plates), to better answer to each of your project issues.

AnyGenes® MICROBIOTA DATABASE

To offer you a complete solution, AnyGenes® has generated a strong database of microbial strains on the basis of high impact factor publications implying high-throughput microbiota analysis. You can search in our database your favorite microbial strains to compose your own Microbiota qPCR Assays and SignArrays®:

Search your favorite bacterial species

Host Species :

Search by :

Search by body site (ecosystem) :




Microbiota individual assays prices

Reference type : MB–XXXX–100
Microbiota individual assays
Number of
Reactions		Price
(before TAX)
100
***
200
***
500
***


*XXXX = microbial species name (according to the NCBI Taxonomy Browser approved nomenclature)

(see Compatibility file)

* required number of reactions

* MB for Microbiota

Example: ME-B.vulgatus.8482-100 for the Bacteroides vulgatus ATCC 8482 species for 100 reactions

Microbiota SignArrays® 96 system prices

Reference type : PZxHMB1-Y2S
Customizable
Number of SignArrays® 96 Price/unit (before TAX)
1-4
***
5-8
***
9-12
***
13-24
***
25-30
***
> 30
***

* Y: R, A, B or F plate type, according to the qPCR instrument (cf Compatibility file)

(see Compatibility file)

*2: according to the number of ordered Microbiota SignArrays®

example: PZxHMB1-R12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument

NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.

Microbiota SignArrays® 384 system prices

Reference type : PZxHMB2-Y2S
Customizable
Number of SignArrays® 384 Price/unit (before TAX)
1-4
**
5-12
***
13-24
***
>24
***

* Y: R, A, B or F plate type, according to the qPCR instrument

(see Compatibility file)

*2: according to the number of ordered Microbiota SignArrays®

example: PZxHMB2-R12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument

** Please request for an estimate at: [email protected]

NB: The minimum order for customizable plates are 2 for less than 32 different species/plate or 6 for more than 32 different species/plate.

Microbiota individual assays, specific of the microbial stains of your choice, are designed and experimentally validated to give you highly reliable results in qPCR.
Analyse the microbiota composition of your samples with the qPCR Microbiota SignArrays 384.
Analyse the microbiota composition of your samples with the qPCR Microbiota SignArrays 384.

ASSOCIATED PMS REAGENTS

PMS Reagents associated with microbiota individual assays prices

Reference type : PMS1-Z100
Perfect Master Mix SYBR® Green reagent (PMS)
Number of Reactions Price (before TAX)
100
***
200
***
500
***

* 10 μl of PMS/reaction for 20 μl final volume

(see Compatibility file)

* Z: W, R, LR or F PMS, according to the qPCR instrument

* required number of reactions

Example: PMS1-W100 for Perfect Master Mix SYBR® Green for 100 reactions with the Light Cycler® 480 instrument

PMS Reagents associated with SignArrays® 96 prices

Reference type : PMS1-Z2S
PMS Perfect Master Mix SYBR® Green SignArrays® 96*
Number of SignArrays® 96 Price/unit (before TAX)
1-4
***
5-8
***
9-12
***
13-24
***
>24
***

* 10 μl of PMS/reaction for 20 μl final volume

(see Compatibility file)

* Z: W, R, LR or F PMS, according to the qPCR instrument

*2: according to the number of ordered Microbiota SignArray®

example: PMS1-W12S for 12 Microbiota SignArrays® 96 for Light Cycler® 480 instrument

PMS Reagents associated with SignArrays® 384 prices

Reference type : PMS2-Z2S
PMS (Perfect Master Mix SYBR® Green) SignArrays® 384*
Number of SignArrays® 384 Price/unit (before TAX)
1-4
***
5-12
***
13-24
***
>24
***

* 5 μl of PMS/reaction for 10 μl final volumes

* Z: W, R, LR or F PMS, according to the qPCR instrument

(see Compatibility file)

*2: according to the number of ordered Microbiota SignArrays®

** Please request for an estimate at: [email protected]

example: PMS2-W12S for 12 Microbiota SignArrays® 384 for Light Cycler® 480 instrument

 
All our assays are validated at the experimental level to guarantee you the best results.