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Direct lysis qPCR reagents for efficient single-cell analysis

AnyGenes® Direct Lysis reagents (DiLysis kits) provide a fast, extraction-free workflow for single-cell RT-qPCR and gene expression analysis.

From as few as 10 cells, you can obtain precise, reproducible, and sensitive results, ideal for transcriptomic profiling and cell signaling pathway studies

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From only few cells, you can now directly analyse signaling pathways by qPCR arrays by using our optimised Direct Lysis reagents (DiLysis kit).
From only few cells, you can now directly analyse signaling pathways by qPCR arrays by using our optimised Diredct Lysis reagents (DiLysis kit)

Simplify your single-cell workflow with direct lysis qPCR

The Direct Lysis qPCR reagents developed by AnyGenes® enable researchers to perform RT-qPCR directly from cell lysates—no RNA extraction required.

This innovative formulation preserves RNA integrity, minimizes sample loss, and accelerates your workflow

For enhanced sensitivity and reproducibility, combine with the SpeAmp® cDNA preamplification kit.

Key benefits

  • Simple and fast protocol
  • Direct cell lysis – no RNA extraction needed
  • Preserve RNA quality and integrity
  • Reduce risk of contamination and loss
  • Fully compatible with RT-qPCR and qPCR arrays
  • Reliable results from as few as 10–100 cells
Direct Lysis reagents (DiLysis kit)have been optimised to offer you a simple and fast procedure to directly proceed to transcriptomic analysis from few cells.
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A reliable and reproducible direct lysis qPCR system

Developed by the AnyGenes® scientific team, the DiLysis kits are widely used in biomarker discovery and single-cell signaling pathway analysis.

Peer-reviewed validation

Multiple studies confirm the robustness and accuracy of direct lysis + RT-qPCR approaches for low-input gene expression profiling :
  • Švec D. et al. Direct Cell Lysis for Single-Cell Gene Expression Profiling, BMC Genomics, 2013 (PMC3819639) (doi: 10.3389/fonc.2013.00274)
  • Ho Y. K. et al. Direct Quantification of mRNA and miRNA from Cell Lysates, PLoS ONE, 2013 (doi:10.1371/journal.pone.0072463)
  • Le A.-P. et al. An Optimized Direct Lysis Method for Gene Expression in Low Cell Numbers, Scientific Reports, 2015 (doi:10.1038/srep12859)
  • …
These studies validate the scientific reliability of direct lysis qPCR workflows for low RNA input and single-cell experiments, in line with the high-performance standards of AnyGenes® DiLysis kits.

Enhanced workflow with SpeAmp®

Combining DiLysis kits with the SpeAmp® cDNA preamplification system allows detection of multiple gene targets even from very low cell numbers.

This powerful workflow enhances the sensitivity and scope of your single-cell RT-qPCR studies.

Applications:

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Technical overview - Direct Lysis qPCR kits

Catalog
Reference		Number of
Reactions		Price
DiLysis-10
10
***
DiLysis-25
25
***
DiLysis-50
50
***

For pricing and distributor information, please contact AnyGenes®.

[email protected]

These prices are available only in FRANCE,

Find a local distributor

Proven expertise in molecular biology and qPCR technologies

With over 20 years of expertise in quantitative real-time PCR, AnyGenes® collaborates with leading academic and clinical research laboratories worldwide.

Our SignArrays®, SpeAmp®, and Perfect Master Mix® technologies are trusted by researchers for their precision, reproducibility, and consistent performance.

Learn more on our Single Cell Profiling

Explore peer-reviewed articles, collaborative research, and citations featuring AnyGenes® technologies in journals such as Nature, PLoS ONE, and Circulation.

Discover our scientific publications

Ready to simplify your single-cell RT-qPCR workflow? Contact our experts to get your customized Direct Lysis (DiLysis) solution today.

Frequently asked questions

Direct lysis reagents are designed to lyse cells directly, eliminating RNA extraction steps before RT-qPCR. They enable fast and efficient gene expression analysis, especially from small cell numbers.

Yes, AnyGenes® Direct Lysis reagents (DiLysis kits) are optimized for single-cell applications, allowing precise transcriptomic profiling from 10 to 100 cells.

The optimized buffer formulation prevents RNA degradation during lysis, ensuring high-quality templates for downstream RT-qPCR.

Combining the Direct Lysis reagents with AnyGenes® SpeAmp® preamplification system enhances detection sensitivity and reproducibility.

Need more details ? Explore our Help & FAQ center for in-depth answers about AnyGenes® products, applications, and technical support.