How AnyGenes® controls the quality of the primers ? In order to perform analysis with our SignArrays® and ensure high quality results,
alEach pair of primers was validated in our laboratory.
Thanks to very stringent design methods, these primers allow a specific,
highly sensitive and reproducible amplification. In fact,
our feature is based on the design of our primers,
which is carried out on two exons, thereby avoiding amplification
of genomic DNA contamination. Upon receipt, a quality control is
performed for all pairs of primers and should therefore ensure reproducible results,
qPCR optimal efficiency and a lack of primer-dimers formation. The specificity of
the primers to their target cDNA sequence is also validated through the presence
of a single peak at the melting curves and a single band on agarose gel corresponding
to the expected size of the amplicon.
What should I do if my sample is contaminated with genomic DNA ? A good qPCR laboratory practices must be respected to avoid any contamination,
ie handle in a space called "DNA-free". Respect the extraction protocol recommended
by the supplier. If traces of genomic DNA exist, we advise you to add a DNAse
treatment step after RNA extraction. Moreover, our design minimizes the genomic
DNA quantification.
What are the quality controls in your SignArrays ? The SignArrays plates have four quality controls:
2 negative controls to ensure the quality of your preparation reaction mix
2 positive controls to check the qPCR performance.
Moreover, a very low value of the positive controls standard deviation allows to check the reproducibility of qPCR reaction with our kit and your samples.
Which type of qPCR instrument should I use with your SignArrays ? Our SignArrays are compatible with most of qPCR instruments.
We will supply the adapted SignArrays plate format, mentioned during your order.
How much does a SignArrays plate cost ? And an analysis in your laboratory ? The price of our SignArrays depends on the amount of ordered plates and
the number of tests you want to perform. We offer very attractive prices and the most competitive of the market.
What would happen if I used my own mix with SignArrays ? We recommend using our SignArrays with our reagents that we have optimized
for this use. However, you can use reagents provided from others suppliers
of your choice, but we could not guarantee the obtained results, as we did
not tested them with our SignArrays.
What types of fluorescent markers do you use in your SignArrays ? Do you use probes ? All plates SignArrays standards are used with SYBR® Green.
However, we are offer SignArrays with specific probes.
How long it takes to perform an analysis ? For already designed signaling pathways,
it will take 1-2 weeks. For personalized SignArrays,
it will take 4-5 weeks. Contact us for large quantity of samples.
What is the amplicons size ? Primers are specifically designated to generate amplicons between 70 and 90 bps.
This design is also adapted for FFPE analysis.
Your products have been referenced in publications? Our reagents Perfect MasterMix SYBR® Green and Perfect MasterMix Probe have already been referenced
in several publications. Several publications are in progress for our SignArrays.
What is the efficiency of qPCR with SignArrays ? Oligos present in our SignArrays enable a qPCR efficiency between 95 and 105% with our Perfect MasterMix.
Sample preparation