You have only few cells and you need more results ?
We have the solution! AnyGenes® set up a new range of kits named Direct Lysis to SignArrays® kits (DiLysis) based on direct lysis of cells optimised to be followed by RT-qPCR (no RNA extraction is needed), which allows you to perform quantitative real-time PCR analysis with only 10 to 100 cells.
Depending on the number and the gene expression profile of analysed factors by qPCR on your cells, this flexible system can allow you to study gene expression of a panel of genes directly without any RNA extraction step.
To optimize your qPCR results, we strongly recommend to combine it with our cDNA pre-amplification SpeAmpn system. All these possibilities have been optimised and the very high performance of all our reagents is controlled with our strict quality control policy to ensure you the best downstream quantitative real-time PCR results (reliability, sensitivity, specificity, repeatability...).
DiLysis reagents contain an optimized lysis buffer to ensure you in only one step:
- direct lysis of your cells without any RNA extraction step
- preservation RNA integrity / avoid RNA degradation
- no loss of biologic material by only step procedure
- direct use for downstream qPCR experiments without any alteration of qPCR performance
Direct Lysis to SignArray® Kits prices